{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:27:46Z","timestamp":1791556066165,"version":"4.3.4"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI132695"],"award-info":[{"award-number":["R01AI132695"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic wasting disease (CWD) is a prionopathy affecting wild and farmed cervids. This disease is endemic in North America and has been recently identified in Europe. Ante-mortem CWD tests of pre-clinical cervids may be an important tool in helping control the spread of this disease. Unfortunately, current CWD diagnostic methods are not suitable for non-tissue type samples. We reported that CWD prions can be detected in blood of pre-clinical CWD-infected white-tailed deer (WTD) with high sensitivity and specificity using the Protein Misfolding Cyclic Amplification (PMCA) assay. However, that report only included animals homozygous for codon 96G, the most common polymorphic version of the prion protein within this animal species. Here, we report CWD prion detection using blood of naturally infected WTD coding one or two copies of the PrP-96S polymorphic variant. Our results, from a blinded screening, show 100% specificity and\u2009~\u200958% sensitivity for animals harboring one 96S codon, regardless of their stage within the pre-clinical phase. Detection efficiency for PrP-96S homozygous animals was substantially lower, suggesting that this allele affect peripheral prion replication\/tropism. These results provide additional information on the influence of codon 96 polymorphisms and the ability of PMCA to detect CWD in the blood of pre-clinical WTD.<\/jats:p>","DOI":"10.1038\/s41598-020-75681-7","type":"journal-article","created":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T11:02:55Z","timestamp":1605265375000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Chronic wasting disease (CWD) prion detection in blood from pre-symptomatic white-tailed deer harboring PRNP polymorphic variants"],"prefix":"10.1038","volume":"10","author":[{"given":"Carlos","family":"Kramm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy A.","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,13]]},"reference":[{"key":"75681_CR1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.7589\/0090-3558-16.1.89","volume":"16","author":"ES Williams","year":"1980","unstructured":"Williams, E. S. & Young, S. Chronic wasting disease of captive mule deer: A spongiform encephalopathy. J. Wildl. Dis. 16, 89\u201398 (1980).","journal-title":"J. Wildl. Dis."},{"key":"75681_CR2","doi-asserted-by":"publisher","DOI":"10.2147\/vmrr.s197404","author":"NA Rivera","year":"2019","unstructured":"Rivera, N. A., Brandt, A. L., Novakofski, J. E. & Mateus-Pinilla, N. E. Chronic wasting disease in cervids: Prevalence, impact and management strategies. Vet. Med. Res. Rep. https:\/\/doi.org\/10.2147\/vmrr.s197404 (2019).","journal-title":"Vet. Med. Res. Rep."},{"key":"75681_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63945-5.00008-8","author":"SL Benestad","year":"2018","unstructured":"Benestad, S. L. & Telling, G. C. Chronic wasting disease: an evolving prion disease of cervids. Handb. Clin. Neurol. https:\/\/doi.org\/10.1016\/B978-0-444-63945-5.00008-8 (2018).","journal-title":"Handb. Clin. Neurol."},{"key":"75681_CR4","doi-asserted-by":"publisher","DOI":"10.1007\/s10344-019-1260-z","author":"A Mysterud","year":"2019","unstructured":"Mysterud, A. & Edmunds, D. R. A review of chronic wasting disease in North America with implications for Europe. Eur. J. Wildl. Res. https:\/\/doi.org\/10.1007\/s10344-019-1260-z (2019).","journal-title":"Eur. J. Wildl. Res."},{"key":"75681_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2013.03.002","author":"N Mateus-Pinilla","year":"2013","unstructured":"Mateus-Pinilla, N., Weng, H. Y., Ruiz, M. O., Shelton, P. & Novakofski, J. Evaluation of a wild white-tailed deer population management program for controlling chronic wasting disease in Illinois, 2003\u20132008. Prev. Vet. Med. https:\/\/doi.org\/10.1016\/j.prevetmed.2013.03.002 (2013).","journal-title":"Prev. Vet. Med."},{"key":"75681_CR6","doi-asserted-by":"publisher","DOI":"10.3996\/032014-JFWM-021","author":"TS Evans","year":"2014","unstructured":"Evans, T. S., Schuler, K. L. & David Walter, W. Surveillance and monitoring of white-tailed deer for chronic wasting disease in the Northeastern United States. J. Fish Wildl. Manage. https:\/\/doi.org\/10.3996\/032014-JFWM-021 (2014).","journal-title":"J. Fish Wildl. Manage."},{"key":"75681_CR7","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1126\/science.1132661","volume":"314","author":"CK Mathiason","year":"2006","unstructured":"Mathiason, C. K. et al. Infectious prions in the saliva and blood of deer with chronic wasting disease. Science 314, 133\u2013136 (2006).","journal-title":"Science"},{"key":"75681_CR8","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1038\/nature08289","volume":"461","author":"G Tamg\u00fcney","year":"2009","unstructured":"Tamg\u00fcney, G. et al. Asymptomatic deer excrete infectious prions in faeces. 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The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"19763"}}