{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:53:24Z","timestamp":1778810004564,"version":"3.51.4"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"crossref","award":["VEGA 1\/0314\/20"],"award-info":[{"award-number":["VEGA 1\/0314\/20"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"crossref","award":["VEGA 1\/0398\/18"],"award-info":[{"award-number":["VEGA 1\/0398\/18"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Canine babesiosis may cause several hematological and biochemical changes, but only limited studies are available regarding the possible differences of changes in animals infected by different <jats:italic>Babesia<\/jats:italic> parasites. The study focused on the evaluation of the differences in serum protein electrophoretic pattern between dogs naturally infected with <jats:italic>B. gibsoni<\/jats:italic> (17 dogs) and <jats:italic>B. canis<\/jats:italic> (40 dogs). The mean values of total proteins, \u03b2<jats:sub>1<\/jats:sub>-, \u03b2<jats:sub>2<\/jats:sub>- and \u03b3-globulins were in dogs infected with <jats:italic>B. gibsoni<\/jats:italic> significantly higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) than in dogs infected with <jats:italic>B. canis<\/jats:italic>. The relative concentrations of albumin, \u03b1<jats:sub>1<\/jats:sub>-, \u03b1<jats:sub>2<\/jats:sub>-globulins and the A\/G ratios were in the <jats:italic>B. gibsoni<\/jats:italic> infected dogs significantly lower (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), no significant differences were found in the relative concentrations of \u03b2<jats:sub>1<\/jats:sub>- and \u03b2<jats:sub>2<\/jats:sub>-globulins. Significant differences were found in most of the evaluated parameters when comparing the results in relation to the form of <jats:italic>B. canis<\/jats:italic> infection to <jats:italic>B. gibsoni<\/jats:italic> infection. Hematological indices showed significant differences between dogs infected with <jats:italic>B. gibsoni<\/jats:italic> and the complicated form of <jats:italic>B. canis<\/jats:italic> infection. In conclusion, the obtained results suggest differences in the changes of serum protein electrophoretic pattern between dogs infected with both <jats:italic>Babesia<\/jats:italic> species and thus, in the response to the infection caused by various <jats:italic>Babesia<\/jats:italic> parasites.<\/jats:p>","DOI":"10.1038\/s41598-020-75908-7","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T11:02:41Z","timestamp":1604401361000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Differences in serum protein electrophoretic pattern in dogs naturally infected with Babesia gibsoni and Babesia canis"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7444-7348","authenticated-orcid":false,"given":"Csilla","family":"T\u00f3thov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Karasov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Bla\u0148arov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1ria","family":"Fialkovi\u010dov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oskar","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"75908_CR1","doi-asserted-by":"publisher","first-page":"225","DOI":"10.17221\/155\/2017-VETMED","volume":"63","author":"L Adaszek","year":"2018","unstructured":"Adaszek, L. et al. The first case of Babesia gibsoni infection in a dog in Poland. Vet. Med. (Czech) 63, 225\u2013228 (2018).","journal-title":"Vet. Med. (Czech)"},{"key":"75908_CR2","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1186\/1756-3305-2-S1-S4","volume":"2","author":"PJ Irwin","year":"2009","unstructured":"Irwin, P. J. Canine babesiosis: from molecular taxonomy to control. Parasites Vectors 2, 54 (2009).","journal-title":"Parasites Vectors"},{"key":"75908_CR3","doi-asserted-by":"publisher","first-page":"59","DOI":"10.4102\/ojvr.v76i1.66","volume":"76","author":"JP Schoeman","year":"2009","unstructured":"Schoeman, J. P. Canine baesiosis. Onderstepoort J. Vet. Res. 76, 59\u201366 (2009).","journal-title":"Onderstepoort J. Vet. Res."},{"key":"75908_CR4","doi-asserted-by":"publisher","first-page":"15","DOI":"10.2149\/tmh1973.6.15","volume":"6","author":"T Ishimine","year":"1978","unstructured":"Ishimine, T., Makimura, S., Kitazawa, S., Tamura, S. & Suzuki, N. Pathophysiological findings on blood of beagles experimentally infected with Babesia gibsoni. Jpn. J. Trop. Med. Hyg. 6, 15\u201326 (1978).","journal-title":"Jpn. J. Tropical Med. Hyg."},{"key":"75908_CR5","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1186\/s13071-016-1596-0","volume":"9","author":"L Solano-Gallego","year":"2016","unstructured":"Solano-Gallego, L., Sainz, A., Roura, X., Estrada-Pe\u00f1a, A. & Mir\u00f3, G. A review of canine-babesiosis: the European perspective. Parasites Vectors 9, 336 (2016).","journal-title":"Parasites Vectors"},{"key":"75908_CR6","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1016\/j.cvsm.2010.08.001","volume":"40","author":"PJ Irwin","year":"2010","unstructured":"Irwin, P. J. Canine babesiosis. Vet. Clin. N. Am. Small Anim. Pract. 40, 1141\u20131156 (2010).","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"75908_CR7","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.vetpar.2011.04.023","volume":"181","author":"L Solano-Gallego","year":"2011","unstructured":"Solano-Gallego, L. & Baneth, G. Babesiosis in dogs and cats\u2014expanding parasitological and clinical spectra. Vet. Parasitol. 181, 48\u201360 (2011).","journal-title":"Vet. Parasitol."},{"key":"75908_CR8","doi-asserted-by":"publisher","first-page":"325","DOI":"10.2460\/javma.2002.220.325","volume":"220","author":"DK MacIntire","year":"2002","unstructured":"MacIntire, D. K. et al. Babesia gibsoni infection among dogs in the southeastern United States. J. Am. Vet. Med. Assoc. 220, 325\u2013329 (2002).","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"75908_CR9","doi-asserted-by":"publisher","first-page":"942","DOI":"10.2460\/javma.2005.227.942","volume":"227","author":"AJ Birkenheuer","year":"2005","unstructured":"Birkenheuer, A. J., Correa, M. T., Levy, M. G. & Breitschwerdt, E. B. Geographic distribution of babesiosis among dogs in the United States and association with dog bites: 150 cases (2000\u20132003). J. Am. Vet. Med. Assoc. 227, 942\u2013947 (2005).","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"75908_CR10","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1111\/j.1751-0813.2007.00220.x","volume":"85","author":"R Jefferies","year":"2007","unstructured":"Jefferies, R. et al. Blood, bull terriers and babesiosis: further evidence for direct transmission of Babesia gibsoni in dogs. Aust. Vet. J. 85, 459\u2013463 (2007).","journal-title":"Aust. Vet. J."},{"key":"75908_CR11","first-page":"156","volume":"72","author":"C Welzl","year":"2011","unstructured":"Welzl, C., Leisewitz, A. L., Jacobson, L. S., Vaughan-Scott, T. & Myburgh, E. Systemic inflammatory response syndrome and multiple-organ damage\/dysfunction in complicated canine babesiosis. J. S. Afr. Vet. Assoc. 72, 156\u2013162 (2011).","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"75908_CR12","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.vetpar.2009.02.012","volume":"162","author":"TPM Schetters","year":"2009","unstructured":"Schetters, T. P. M. et al. Systemic inflammatory responses in dogs experimentally infected with Babesia canis; a haematological study. Vet. Parasitol. 162, 7\u201315 (2009).","journal-title":"Vet. Parasitol."},{"key":"75908_CR13","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1111\/j.1939-165X.2005.tb00028.x","volume":"34","author":"B Ulutas","year":"2005","unstructured":"Ulutas, B., Bayramli, G., Ulutas, P. A. & Karagenc, T. Serum concentration of some acute phase proteins in naturally occurring canine babesiosis: a preliminary study. Vet. Clin. Pathol. 34, 144\u2013147 (2005).","journal-title":"Vet. Clin. Pathol."},{"key":"75908_CR14","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.vetpar.2006.10.004","volume":"144","author":"V Matijatko","year":"2007","unstructured":"Matijatko, V. et al. Evidence of an acute phase response in dogs naturally infected with Babesia canis. Vet. Parasitol. 144, 242\u2013250 (2007).","journal-title":"Vet. Parasitol."},{"key":"75908_CR15","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.exppara.2015.08.002","volume":"157","author":"AL Brown","year":"2015","unstructured":"Brown, A. L., Shiel, R. E. & Irwin, P. J. Clinical, haematological, cytokine and acute phase protein changes during experimental Babesia gibsoni infection of beagle puppies. Exp. Parasitol. 157, 185\u2013196 (2015).","journal-title":"Exp. Parasitol."},{"key":"75908_CR16","doi-asserted-by":"publisher","first-page":"425","DOI":"10.2754\/avb201988040425","volume":"88","author":"C T\u00f3thov\u00e1","year":"2019","unstructured":"T\u00f3thov\u00e1, C. et al. The electrophoretic pattern of serum proteins in dogs with babesiosis. Acta Vet. (Brno) 88, 425\u2013432 (2019).","journal-title":"Acta Vet. (Brno)"},{"key":"75908_CR17","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1089\/vbz.2006.0580","volume":"7","author":"K Hartelt","year":"2007","unstructured":"Hartelt, K. et al. First evidence of Babesia gibsoni (Asian genotype) in dogs in Western Europe. Vector Borne Zoonotic Dis. 7, 163\u2013166 (2007).","journal-title":"Vector Borne Zoonotic Dis."},{"key":"75908_CR18","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1556\/avet.2015.017","volume":"63","author":"D Davitkov","year":"2015","unstructured":"Davitkov, D. et al. Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia. Acta Vet. Hung. 63, 199\u2013208 (2015).","journal-title":"Acta Vet. Hung."},{"key":"75908_CR19","doi-asserted-by":"publisher","first-page":"185","DOI":"10.2460\/javma.2002.220.185","volume":"220","author":"JH Meinkoth","year":"2002","unstructured":"Meinkoth, J. H., Kocan, A. A., Loud, S. D. & Lorenz, M. D. Clinical and hematologic effects of experimental infection in dogs with recently identified Babesia gibsoni-like isolates from Oklahoma. J. Am. Vet. Med. Assoc. 220, 185\u2013189 (2002).","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"75908_CR20","first-page":"119","volume":"6","author":"LS K\u00f6ster","year":"2015","unstructured":"K\u00f6ster, L. S., Lobetti, R. G. & Kelly, P. Canine babesiosis: a\u00a0perspective on clinical complications, biomarkers, and treatment. Vet. Med. Res. Rep. 6, 119\u2013128 (2015).","journal-title":"Vet. Med. Res. Rep."},{"key":"75908_CR21","first-page":"33","volume":"27","author":"L Boozer","year":"2005","unstructured":"Boozer, L. & Macintire, D. Babesia gibsoni: an emerging pathogen in dogs. Comp. Cont. Educ. Pract. Vet. 27, 33\u201342 (2005).","journal-title":"Comp. Cont. Educ. Pract. Vet."},{"key":"75908_CR22","doi-asserted-by":"publisher","first-page":"692","DOI":"10.2307\/3284248","volume":"83","author":"EJ Wozniak","year":"1997","unstructured":"Wozniak, E. J. et al. Clinical, anatomic, and immunopathologic characterization of Babesia gibsoni infection in the domestic dog (Canis familiaris). J. Parasitol. 83, 692\u2013699 (1997).","journal-title":"J. Parasitol."},{"key":"75908_CR23","doi-asserted-by":"publisher","first-page":"127","DOI":"10.2478\/acve-2018-0011","volume":"68","author":"P Bili\u0107","year":"2018","unstructured":"Bili\u0107, P., Kule\u0161, J., Bari\u0107 Rafaj, R. & Mrljak, V. Canine babesiosis: where do we stand?. Acta Vet. (Beograd) 68, 127\u2013160 (2018).","journal-title":"Acta Vet. (Beograd)"},{"key":"75908_CR24","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.vetpar.2005.07.016","volume":"134","author":"T Furlanello","year":"2005","unstructured":"Furlanello, T., Fiorioa, F., Caldina, M., Lubasb, G. & Solano-Gallegoa, L. Clinicopathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy. Vet. Parasitol. 134, 77\u201385 (2005).","journal-title":"Vet. Parasitol."},{"key":"75908_CR25","first-page":"283","volume":"49","author":"IV Maele","year":"2008","unstructured":"Maele, I. V., Bataille, K. S., Gielen, I. & Darninet, S. An unusual form of canine babesiosis. Can. Vet. J. 49, 283\u2013286 (2008).","journal-title":"Can. Vet. J."},{"key":"75908_CR26","first-page":"25","volume":"SP4","author":"P Kumar","year":"2018","unstructured":"Kumar, P. & Kumar, A. Haemato-biochemical changes in dogs infected with Babesiosis. Int. J. Chem. Stud. SP4, 25\u201328 (2018).","journal-title":"Int. J. Chem. Stud."},{"key":"75908_CR27","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/0014-4894(71)90102-0","volume":"30","author":"M Ristic","year":"1971","unstructured":"Ristic, M., Lykins, J. D. & Smith, A. R. Babesia canis and Babesia gibsoni, soluble and corpuscular antigen isolated from blood of dogs. Exp. Parasitol. 30, 385\u2013392 (1971).","journal-title":"Exp. Parasitol."},{"key":"75908_CR28","doi-asserted-by":"publisher","first-page":"247","DOI":"10.12935\/jvma1951.30.247","volume":"30","author":"R Noda","year":"1977","unstructured":"Noda, R. Canine babesiosis. J. Jpn. Vet. Med. Assoc. 30, 247\u2013251 (1977).","journal-title":"J. Jpn. Vet. Med. Assoc."},{"key":"75908_CR29","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.14202\/vetworld.2017.1227-1232","volume":"10","author":"AK Bilwal","year":"2017","unstructured":"Bilwal, A. K., Mandali, G. C. & Tandel, F. B. Clinicopathological alterations in naturally occurring Babesia gibsoni infection in dogs of Middle-South Gujarat, India. Vet. World 10, 1227\u20131232 (2017).","journal-title":"Vet. World"},{"key":"75908_CR30","first-page":"1334","volume":"6","author":"S Yogeshpriya","year":"2018","unstructured":"Yogeshpriya, S. et al. Clinical, haemato-biochemical and ultrasonographical studies on naturally occurring Babesia gibsoni infection in dogs. J. Entomol. Zool. Stud. 6, 1334\u20131337 (2018).","journal-title":"J. Entomol. Zool. Stud."},{"key":"75908_CR31","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1007\/s12639-014-0491-x","volume":"40","author":"BS Reddy","year":"2016","unstructured":"Reddy, B. S., Sivajothi, S., Reddy, L. S. S. & Raju, K. G. S. Clinical and laboratory findings of Babesia infection in dogs. J. Parasit. Dis. 40, 268\u2013272 (2016).","journal-title":"J. Parasit. Dis."},{"key":"75908_CR32","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1159\/000212077","volume":"79","author":"SR Vavricka","year":"2009","unstructured":"Vavricka, S. R., Burri, E., Beglinger, C., Degen, L. & Manz, M. Serum protein electrophoresis: an underused but very useful test. Digestion 79, 203\u2013210 (2009).","journal-title":"Digestion"},{"key":"75908_CR33","first-page":"3","volume":"5","author":"R Yadav","year":"2011","unstructured":"Yadav, R., Gattani, A., Gupta, S. R. & Sharma, C. S. Jaundice in dogs associated with babesiosis\u2014a case report. Int. J. Agric. Vet. Med. Sci. 5, 3\u20136 (2011).","journal-title":"Int. J. Agric. Vet. Med. Sci."},{"key":"75908_CR34","first-page":"21","volume":"91","author":"P Vijayalakshmi","year":"2014","unstructured":"Vijayalakshmi, P. et al. Clinico pathological features in dogs associated with babesiosis. Indian Vet. J. 91, 21\u201324 (2014).","journal-title":"Indian Vet. J."},{"key":"75908_CR35","first-page":"663","volume":"53","author":"W Zygner","year":"2009","unstructured":"Zygner, W. & Wedrychowicz, H. Influence of anemia on azotaemia in dogs infected with Babesia canis in Poland. Bull. Vet. Inst. Pulawy 53, 663\u2013668 (2009).","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"75908_CR36","first-page":"21","volume-title":"Acute Phase Proteins. Molecular Biology, Biochemistry, and Clinical Applications","author":"AR Aldred","year":"1993","unstructured":"Aldred, A. R. & Schreiber, G. The Negative Acute Phase Protein. In Acute Phase Proteins. Molecular Biology, Biochemistry, and Clinical Applications (eds Mackiewicz, I. et al.) 21\u201337 (CRC Press, Boca Raton, 1993)."},{"key":"75908_CR37","first-page":"485","volume":"8","author":"B Maegraith","year":"1957","unstructured":"Maegraith, B., Gilles, H. M. & Devakul, K. Pathological processes in Babesia canis infections. Z. Tropenmed. Parasitol. 8, 485\u2013514 (1957).","journal-title":"Z. Tropenmed. Parasitol."},{"key":"75908_CR38","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1080\/00034983.1965.11686293","volume":"59","author":"A Tella","year":"1965","unstructured":"Tella, A. & Maegraith, B. G. Physiopathological changes in primary acute blood-transmitted malaria and Babesia infections. II. A comparative study of serum protein levels in infected rhesus monkeys, mice and puppies. Ann. Trop. Med. Parasitol. 59, 153\u2013158 (1965).","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"75908_CR39","doi-asserted-by":"crossref","first-page":"38","DOI":"10.4102\/jsava.v71i1.675","volume":"71","author":"RG Lobetti","year":"2000","unstructured":"Lobetti, R. G., Mohr, A. J., Dippenaar, T. & Myburgh, E. A preliminary study on the serum protein response in canine babesiosis. J. S. Afr. Vet. Assoc. 71, 38\u201342 (2000).","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"75908_CR40","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1051\/vetres:2005026","volume":"36","author":"AT Camacho","year":"2005","unstructured":"Camacho, A. T. et al. Serum protein response and renal failure in canine Babesia annae infection. Vet. Res. 36, 713\u2013722 (2005).","journal-title":"Vet. Res."},{"key":"75908_CR41","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1111\/jvim.12146","volume":"27","author":"R Bari\u0107 Rafaj","year":"2013","unstructured":"Bari\u0107 Rafaj, R. et al. Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis. J. Vet. Int. Med. 27, 1172\u20131178 (2013).","journal-title":"J. Vet. Int. Med."},{"key":"75908_CR42","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1136\/vr.d2689","volume":"168","author":"JJ Ceron","year":"2011","unstructured":"Ceron, J. J., Caldin, M. & Martinez-Subiela, S. Answers to some common questions on serum protein electrophoresis. Vet. Rec. 168, 453\u2013454 (2011).","journal-title":"Vet. Rec."},{"key":"75908_CR43","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1053\/tvjl.2001.0672","volume":"164","author":"S Martinez-Subiela","year":"2002","unstructured":"Martinez-Subiela, S., Tecles, F., Montes, A., Guti\u00e9rrez, C. & Cer\u00f3n, J. J. Effects of haemolysis, lipaemia, bilirubinaemia, and fibrinogen on protein electrophoretogram of canine samples analysed by capillary zone electrophoresis. Vet. J. 164, 261\u2013268 (2002).","journal-title":"Vet. J."},{"key":"75908_CR44","first-page":"59","volume":"55","author":"W Zygner","year":"2011","unstructured":"Zygner, W., G\u00f3jska-Zygner, O. & W\u1eb9drychowicz, H. Abnormalities in serum proteins in the course of babesiosis in dogs. Bull. Vet. Inst. Pulawy 55, 59\u201365 (2011).","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"75908_CR45","first-page":"245","volume":"10","author":"W Zygner","year":"2007","unstructured":"Zygner, W., Rapacka, G., G\u00f3jska-Zygner, O., Dlugosz, E. & W\u1eb9drychowicz, H. Biochemical abnormalities observed in serum of dogs infected with large Babesia in Warsaw (Poland). Pol. J. Vet. Sci. 10, 245\u2013253 (2007).","journal-title":"Pol. J. Vet. Sci."},{"key":"75908_CR46","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/1746-6148-10-111","volume":"10","author":"J Kule\u0161","year":"2014","unstructured":"Kule\u0161, J. et al. Identification of serum biomarkers in dogs naturally infected with Babesia canis canis using a proteomic approach. BMC Vet. Res. 10, 111\u2013120 (2014).","journal-title":"BMC Vet. Res."},{"key":"75908_CR47","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/BF00931896","volume":"79","author":"RH Jacobson","year":"1993","unstructured":"Jacobson, R. H., Parrodi, F., Wright, I. G., Fitzgerald, C. J. & Dobson, C. Babesia bovis: in vitro phagocytosis promoted by immune serum and and by antibodies produced against protective antigens. Parasitol. Res. 79, 221\u2013226 (1993).","journal-title":"Parasitol. Res."},{"key":"75908_CR48","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/0165-2427(92)90103-W","volume":"30","author":"S Yamamoto","year":"1992","unstructured":"Yamamoto, S. et al. Isolation of canine C-reactive protein characterization of its properties. Vet. Immunol. Immunopathol. 30, 329\u2013399 (1992).","journal-title":"Vet. Immunol. Immunopathol."},{"key":"75908_CR49","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.tvjl.2010.04.009","volume":"185","author":"PD Eckersall","year":"2010","unstructured":"Eckersall, P. D. & Bell, R. Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet. J. 185, 23\u201327 (2010).","journal-title":"Vet. J."},{"key":"75908_CR50","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/B978-012396305-5\/50006-3","volume-title":"Clinical Biochemistry of Domestic Animals","author":"JJ Kaneko","year":"1997","unstructured":"Kaneko, J. J. Serum Proteins and the Dysproteinemias. In Clinical Biochemistry of Domestic Animals 5th edn (ed. Kaneko, J. J.) 117\u2013138 (Academic Press, London, 1997).","edition":"5"},{"key":"75908_CR51","first-page":"426","volume-title":"Large Animal Internal Medicine","author":"D Morris","year":"2002","unstructured":"Morris, D. & Johnston, J. K. Alterations in Blood Proteins. In Large Animal Internal Medicine 3rd edn (ed. Smith, B. P.) 426\u2013433 (Mosby, St. Louis, 2002).","edition":"3"},{"key":"75908_CR52","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1136\/vr.150.8.245","volume":"150","author":"S Okano","year":"2002","unstructured":"Okano, S. et al. Usefulness of systemic inflammatory response syndrome criteria as an index for prognosis judgement. Vet. Rec. 150, 245\u2013246 (2002).","journal-title":"Vet. Rec."},{"key":"75908_CR53","first-page":"65","volume":"13","author":"S Casati","year":"2006","unstructured":"Casati, S., Sager, H., Gern, L. & Piffaretti, J. C. Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Ann. Agric. Environ. Med. 13, 65\u201370 (2006).","journal-title":"Ann. Agric. Environ. Med."},{"key":"75908_CR54","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1292\/jvms.67.467","volume":"67","author":"T Miyama","year":"2005","unstructured":"Miyama, T. et al. Epidemiological survey of Babesia gibsoni infection in dogs in eastern. Jpn J. Vet. Med. Sci. 67, 467\u2013471 (2005).","journal-title":"Jpn J. Vet. Med. Sci."},{"key":"75908_CR55","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1093\/molbev\/mst197","volume":"30","author":"K Tamura","year":"2013","unstructured":"Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725\u20132729 (2013).","journal-title":"Mol. Biol. Evol."},{"key":"75908_CR56","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"SF Altschul","year":"1997","unstructured":"Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389\u20133402 (1997).","journal-title":"Nucleic Acids Res."},{"key":"75908_CR57","doi-asserted-by":"publisher","first-page":"187","DOI":"10.2754\/avb201584020187","volume":"84","author":"O Nagy","year":"2015","unstructured":"Nagy, O., T\u00f3thov\u00e1, C., Nagyov\u00e1, V. & Kov\u00e1\u010d, G. Comparison of serum protein electrophoretic pattern in cows and small ruminants. Acta Vet. (Brno) 84, 187\u2013195 (2015).","journal-title":"Acta Vet. (Brno)"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-75908-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-75908-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-75908-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T22:38:05Z","timestamp":1670366285000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-75908-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,3]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["75908"],"URL":"https:\/\/doi.org\/10.1038\/s41598-020-75908-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,3]]},"assertion":[{"value":"25 August 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 November 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"18904"}}