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Canine brucellosis is characterized by reproductive failure in dogs. Human\n                    <jats:italic>Brucella canis<\/jats:italic>\n                    infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption\/ionization\u2013time of flight mass spectrometry analysis was applied, which allowed for rapid identification of\n                    <jats:italic>B. canis<\/jats:italic>\n                    and differentiation from closely related\n                    <jats:italic>B. suis<\/jats:italic>\n                    biovar 1. High-throughput sequencing and comparative genomics using single nucleotide polymorphism analysis clustered our isolates together with canine and human strains from various Central and South American countries in a distinct sub-lineage. Hence, molecular epidemiology clearly defined the outbreak cluster and demonstrated the endemic situation in South America. Our study illustrates that MALDI-TOF MS analysis using a validated in-house reference database facilitates rapid\n                    <jats:italic>B. canis<\/jats:italic>\n                    identification at species level. Additional whole genome sequencing provides more detailed outbreak information and leads to a deeper understanding of the epidemiology of canine brucellosis.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-75960-3","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T06:03:58Z","timestamp":1604642638000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks"],"prefix":"10.1038","volume":"10","author":[{"given":"David Attuy Vey","family":"da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Brendebach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Gr\u00fctzke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Dieckmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Martins","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Teresa Ribeiro","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara Borges","family":"Keid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Hofreuter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Al Dahouk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,6]]},"reference":[{"key":"75960_CR1","doi-asserted-by":"publisher","first-page":"289","DOI":"10.3325\/cmj.2010.51.289","volume":"51","author":"M Gwida","year":"2010","unstructured":"Gwida, M. et al. 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