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This research aims to study the anti-aging effects of GRCP, a compound preparation made by\n                    <jats:italic>Ganoderma lucidum<\/jats:italic>\n                    and\n                    <jats:italic>Rhodiola rosen<\/jats:italic>\n                    , in aging rats. Rats were subcutaneously injected with 400\u00a0mg\/kg of\n                    <jats:sc>d<\/jats:sc>\n                    -galactose daily, and aging could be induced after 8\u00a0weeks. The aging rats were treated with GRCP. This experiment was divided into 6 groups. Rats were randomly divided into the model group, positive control group, low-dose GRCP group (25\u00a0mg\/kg body weight), medium-dose GRCP group (50\u00a0mg\/kg body weight), and high-dose GRCP group (100\u00a0mg\/kg body weight), healthy and normal rats were used as blank controls. After the end, the results show that the use of GRCP at a dose of 100\u00a0mg\/kg is the best treatment for improving aging rats. Rats gained weight, spleen and thymus indexes, and splenocyte proliferation improved, and inflammatory cytokine levels decreased. Besides, biochemical indicators show that GRCP can improve the antioxidant enzyme activity and reduce the content of lipofuscin and TGF-\u03b2 in aging rats (P\u2009&lt;\u20090.05). GRCP can also inhibit the activation of the MyD88\/NF-\u03baB pathway in rat hippocampus. These results seem to suggest that GRCP can be used as a potential natural supplement or functional food to prevent aging.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-76249-1","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T06:02:46Z","timestamp":1604642566000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Ganoderma lucidum Rhodiola compound preparation prevent d-galactose-induced immune impairment and oxidative stress in aging rat model"],"prefix":"10.1038","volume":"10","author":[{"given":"Shuo","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Do","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinggao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,6]]},"reference":[{"key":"76249_CR1","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1083\/jcb.201708092","volume":"217","author":"D McHugh","year":"2018","unstructured":"McHugh, D. & Gil, J. 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