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Here, we show that the intracellular pH of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    equilibrates to the environmental pH following treatment with the DNA damaging antibiotic nalidixic acid. We demonstrate that this allows the environmental pH to influence the transcription of various DNA damage response genes and physiological processes such as filamentation. Using purified RecA and a known pH-sensitive mutant variant RecA K250R we show how pH can affect the biochemical activity of a protein central to control of the bacterial DNA damage response system. Finally, two different mutagenesis assays indicate that environmental pH affects antibiotic resistance development. Specifically, at environmental pH\u2019s greater than six we find that mutagenesis plays a significant role in producing antibiotic resistant mutants. At pH\u2019s less than or equal to 6 the genome appears more stable but extensive filamentation is observed, a phenomenon that has previously been linked to increased survival in the presence of macrophages.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-76426-2","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T06:02:41Z","timestamp":1604988161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability"],"prefix":"10.1038","volume":"10","author":[{"given":"James Alexander","family":"Booth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio","family":"\u0160p\u00edrek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tekle Airgecho","family":"Lobie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Skarstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumir","family":"Krejci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnar","family":"Bj\u00f8r\u00e5s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"76426_CR1","unstructured":"EU, F. 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