{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:00:49Z","timestamp":1787320849277,"version":"build-2736575974"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K09716"],"award-info":[{"award-number":["16K09716"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H06277"],"award-info":[{"award-number":["16H06277"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18H02741"],"award-info":[{"award-number":["18H02741"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19dm0107106"],"award-info":[{"award-number":["JP19dm0107106"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Lewy bodies (LBs) and glial cytoplasmic inclusions (GCIs) are specific aggregates found in Parkinson\u2019s disease (PD) and multiple system atrophy (MSA), respectively. These aggregates mainly consist of \u03b1-synuclein (\u03b1-syn) and have been reported to propagate in the brain. In animal experiments, the fibrils of \u03b1-syn propagate similarly to prions but there is still insufficient evidence to establish this finding in humans. Here, we analysed the protein structure of these aggregates in the autopsy brains of patients by synchrotron Fourier-transform infrared micro-spectroscopy (FTIRM) analysis without extracting or artificially amplifying the aggregates. As a result, we found that the content of the \u03b2-sheet structure in LBs in patients with PD was significantly higher than that in GCIs in patients with MSA (52.6\u2009\u00b1\u20091.9% in PD vs. 38.1\u2009\u00b1\u20090.9% in MSA,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001). These structural differences may provide clues to the differences in phenotypes of PD and MSA.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-76565-6","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T06:02:41Z","timestamp":1604988161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The secondary structural difference between Lewy body and glial cytoplasmic inclusion in autopsy brain with synchrotron FTIR micro-spectroscopy"],"prefix":"10.1038","volume":"10","author":[{"given":"Katsuya","family":"Araki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Yagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Ikemoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hayakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harutoshi","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Moriwaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Murayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Mochizuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"76565_CR1","doi-asserted-by":"publisher","first-page":"6469","DOI":"10.1073\/pnas.95.11.6469","volume":"95","author":"MG Spillantini","year":"1998","unstructured":"Spillantini, M. G., Crowther, R. A., Jakes, R., Hasegawa, M. & Goedert, M. \u03b1-Synuclein in filamentous inclusions of Lewy bodies from Parkinson disease and dementia with Lewy bodies. Proc. Natl. Acad. Sci. 95, 6469\u20136473 (1998).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"76565_CR2","first-page":"879","volume":"152","author":"M Baba","year":"1998","unstructured":"Baba, M. et al. Aggregation of \u03b1-synuclein in Lewy bodies of sporadic Parkinson disease and dementia with Lewy bodies. Am. J. Pathol. 152, 879\u2013884 (1998).","journal-title":"Am. J. Pathol."},{"key":"76565_CR3","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/S0304-3940(98)00504-7","volume":"251","author":"MG Spillantini","year":"1998","unstructured":"Spillantini, M. G. et al. Filamentous alpha-synuclein inclusions link multiple system atrophy with Parkinson\u2019s disease and dementia with Lewy bodies. Neurosci. Lett. 251, 205\u2013208 (1998).","journal-title":"Neurosci. Lett."},{"key":"76565_CR4","doi-asserted-by":"publisher","first-page":"E5308","DOI":"10.1073\/pnas.1514475112","volume":"112","author":"SB Prusiner","year":"2015","unstructured":"Prusiner, S. B. et al. Evidence for \u03b1-synuclein prions causing multiple system atrophy in humans with parkinsonism. Proc. Natl. Acad. Sci. USA 112, E5308-5317 (2015).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"76565_CR5","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1126\/science.1227157","volume":"338","author":"KC Luk","year":"2012","unstructured":"Luk, K. C. et al. Pathological \u03b1-synuclein transmission initiates Parkinson-like neurodegeneration in nontransgenic mice. Science 338, 949\u2013953 (2012).","journal-title":"Science"},{"key":"76565_CR6","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1093\/brain\/awt037","volume":"136","author":"M Masuda-Suzukake","year":"2013","unstructured":"Masuda-Suzukake, M. et al. Prion-like spreading of pathological \u03b1-synuclein in brain. Brain 136, 1128\u20131138 (2013).","journal-title":"Brain"},{"key":"76565_CR7","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1039\/c3an00295k","volume":"138","author":"CR Liao","year":"2013","unstructured":"Liao, C. R. et al. Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer\u2019s disease brain. Analyst. 138, 3991\u20133997 (2013).","journal-title":"Analyst."},{"key":"76565_CR8","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.1016\/S0006-3495(96)79411-0","volume":"71","author":"LP Choo","year":"1996","unstructured":"Choo, L. P. et al. In situ characterization of beta-amyloid in Alzheimer\u2019s diseased tissue by synchrotron Fourier transform infrared microspectroscopy. Biophys. J. 71, 1672\u20131679 (1996).","journal-title":"Biophys. J."},{"key":"76565_CR9","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.jsb.2005.09.004","volume":"155","author":"LM Miller","year":"2006","unstructured":"Miller, L. M. et al. Synchrotron-based infrared and X-ray imaging shows focalized accumulation of Cu and Zn co-localized with beta-amyloid deposits in Alzheimer\u2019s disease. J. Struct. Biol. 155, 30\u201337 (2006).","journal-title":"J. Struct. Biol."},{"key":"76565_CR10","doi-asserted-by":"publisher","first-page":"17625","DOI":"10.1038\/srep17625","volume":"5","author":"K Araki","year":"2015","unstructured":"Araki, K. et al. Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson\u2019s disease patients. Sci. Rep. 5, 17625 (2015).","journal-title":"Sci. Rep."},{"key":"76565_CR11","doi-asserted-by":"publisher","first-page":"2910","DOI":"10.1002\/jps.20890","volume":"96","author":"S Luthra","year":"2007","unstructured":"Luthra, S., Kalonia, D. S. & Pikal, M. J. Effect of hydration on the secondary structure of lyophilized proteins as measured by Fourier transform infrared (FTIR) spectroscopy. J. Pharm. Sci. 96, 2910\u20132921 (2007).","journal-title":"J. Pharm. Sci."},{"key":"76565_CR12","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1366\/000370209787944316","volume":"63","author":"A Sachdeva","year":"2009","unstructured":"Sachdeva, A. & Cai, S. Structural differences of proteins between solution state and solid state probed by attenuated total reflection Fourier transform infrared spectroscopy. Appl. Spectrosc. 63, 458\u2013464 (2009).","journal-title":"Appl. Spectrosc."},{"key":"76565_CR13","doi-asserted-by":"publisher","first-page":"17963","DOI":"10.1073\/pnas.1906124116","volume":"116","author":"K Araki","year":"2019","unstructured":"Araki, K. et al. Parkinson\u2019s disease is a type of amyloidosis featuring accumulation of amyloid fibrils of \u03b1-synuclein. Proc. Natl. Acad. Sci. 116, 17963\u201317969 (2019).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"76565_CR14","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1038\/s41586-020-2317-6","volume":"585","author":"M Schweighauser","year":"2020","unstructured":"Schweighauser, M. et al. Structures of \u03b1-synuclein filaments from multiple system atrophy. Nature 585, 464\u2013469 (2020).","journal-title":"Nature"},{"key":"76565_CR15","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1038\/s41586-020-1984-7","volume":"578","author":"M Schweighauser","year":"2020","unstructured":"Schweighauser, M. et al. Discriminating \u03b1-synuclein strains in Parkinson\u2019s disease and multiple system atrophy. Nature 578, 273\u2013277 (2020).","journal-title":"Nature"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-76565-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-76565-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-76565-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T17:33:17Z","timestamp":1670347997000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-76565-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,10]]},"references-count":15,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["76565"],"URL":"https:\/\/doi.org\/10.1038\/s41598-020-76565-6","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,10]]},"assertion":[{"value":"24 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 November 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"19423"}}