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An effective control and management of MSVD requires robust and sensitive\u00a0diagnostic tests capable of rapid detection of MSV. In this study, a loop-mediated isothermal amplification (LAMP) assay was designed for the specific detection of MSV. This test has shown to be highly specific and reproducible and able to detect MSV in as little as 10\u00a0fg\/\u00b5l of purified genomic DNA obtained from a MSV-infected maize plant, a sensitivity 10<jats:sup>5<\/jats:sup> times higher to that obtained with polymerase chain reaction (PCR) in current general use. The high degree of sequence identity between Zambian and other African MSV isolates indicate that this LAMP assay can be used for detecting MSV in maize samples from any region in Africa. Furthermore, this assay can be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories across Africa strengthening diagnostic capacity in countries dealing with MSD.<\/jats:p>","DOI":"10.1038\/s41598-020-76612-2","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T11:02:41Z","timestamp":1605178961000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A quick and sensitive diagnostic tool for detection of Maize streak virus"],"prefix":"10.1038","volume":"10","author":[{"given":"Mathias","family":"Tembo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adedapo O.","family":"Adediji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Bouvaine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C.","family":"Chikoti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan E.","family":"Seal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gon\u04abalo","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"76612_CR1","doi-asserted-by":"publisher","first-page":"406","DOI":"10.2134\/agronj2015.0540","volume":"109","author":"PS Setimela","year":"2017","unstructured":"Setimela, P. 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