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We investigated the effect of a single dose of 100,000\u00a0IU of cholecalciferol, in office blood pressure (BP), and 24-h ambulatory blood pressure monitoring (ABPM) in patients with type 2 diabetes mellitus (DM), hypertension, and hypovitaminosis D. Forty-three patients were randomized to a placebo or cholecalciferol group. BP was assessed by office measurements and 24-h ABPM, before and after intervention. At week 8, a greater decrease in median ABPM values was observed in cholecalciferol supplementation than in the placebo group for systolic 24-h (\u2212\u00a07.5 vs. \u2212\u00a01; <jats:italic>P<\/jats:italic>\u2009=\u20090.02), systolic daytime (\u2212\u00a07 vs. \u2212\u00a01; <jats:italic>P<\/jats:italic>\u2009=\u20090.007), systolic nighttime (\u2212\u00a07.0 vs. 3; <jats:italic>P<\/jats:italic>\u2009=\u20090.009), diastolic 24-h (\u2212\u00a03.5 vs. \u2212\u00a01; <jats:italic>P<\/jats:italic>\u2009=\u20090.037), and daytime DBP (\u2212\u00a05 vs. 0; <jats:italic>P<\/jats:italic>\u2009=\u20090.01). Office DBP was also reduced after vitamin D supplementation. A single dose of vitamin D<jats:sub>3<\/jats:sub> improves BP in patients with type 2 diabetes, hypertension, and vitamin D insufficiency, regardless of vitamin D normalization. Vitamin D supplementation could be a valuable tool to treat patients with type 2 DM, hypertension, and hypovitaminosis D.<\/jats:p><jats:p><jats:bold>Trial registration<\/jats:bold>: Clinicaltrials.gov NCT 02204527.<\/jats:p>","DOI":"10.1038\/s41598-020-76646-6","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T11:03:11Z","timestamp":1605178991000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Efficacy of single-dose cholecalciferol in the blood pressure of patients with type 2 diabetes, hypertension and hypovitaminoses D"],"prefix":"10.1038","volume":"10","author":[{"given":"Tatiana P.","family":"de Paula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliano S. 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