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Fatty acids were extracted from frozen sperm in 1:2 (v\/v) chloroform\u2013methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography\u2013mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18\u00a0\u00b5g) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9\u00a0\u00b5g;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (\n                    <jats:italic>P<\/jats:italic>\n                    \u2264 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-77089-9","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T06:03:51Z","timestamp":1605765831000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Lipidomic markers of sperm cryotolerance in cattle"],"prefix":"10.1038","volume":"10","author":[{"given":"Holly C.","family":"Evans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thu T. 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