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The etiology is believed to be multifactorial and alterations have been documented in the biomechanical, biochemical and ultrastructural characteristics of the cornea. While the exact site of disease\u00a0origin is still debated, changes in the corneal epithelium are believed to occur even before the disease is clinically manifested. In this study we investigate the possible role of \u03b2-catenin as mechanotransducer in KC corneal epithelium. The sheets of corneal epithelium removed from keratoconic eyes when they underwent collagen crosslinking as a therapeutic procedure were used for this study. The healthy corneal epithelium of patients undergoing Laser Refractive Surgery for the correction of their refractive error, served as controls. Immunoblotting and tissue immunofluorescence studies were performed on KC epithelium to analyse the expression and localization of \u03b2-catenin, E-cadherin, ZO1, \u03b1-catenin, Cyclin D1, \u03b1-actinin, RhoA, and\u00a0Rac123. Co-immunoprecipitation of \u03b2-catenin followed by mass spectrometry of KC epithelium was performed to identify its interacting partners. This was further validated by using epithelial tissues grown on scaffolds of different stiffness. Histology data reported breaks in the Bowman\u2019s layer in KC patients. We hypothesize that these breaks expose the epithelium to the keratoconic\u00a0corneal stroma, which, is known to have a decreased elastic modulus and that \u03b2-catenin acts as a mechanotransducer that induces structural changes such as loss of polarity (Syntaxin3) and barrier function (ZO1) through membrane delocalization. The results of our study strongly suggest that \u03b2-catenin could be a putative mechanotransducer in KC epithelium, thus supporting our hypothesis.<\/jats:p>","DOI":"10.1038\/s41598-020-77138-3","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T06:07:06Z","timestamp":1607321226000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Deciphering the mechanoresponsive role of \u03b2-catenin in keratoconus epithelium"],"prefix":"10.1038","volume":"10","author":[{"given":"Chatterjee","family":"Amit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prema","family":"Padmanabhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0866-9828","authenticated-orcid":false,"given":"Janakiraman","family":"Narayanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"77138_CR1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1038\/nrm2592","volume":"10","author":"MA Wozniak","year":"2009","unstructured":"Wozniak, M. 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