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In chronic viral infections, the deranged B cell subset signifies uncontrolled disease. The study aimed to verify the relation between B cell subsets\u2019 distribution and liver disease progression in chronic hepatitis C (CHC) patients with T2D. A total of 67 CHC patients were divided into two groups; 33 non-diabetic and 34 with T2D. Each group was subdivided into CHC-without LC or HCC (N-CHC), CHC-with LC (CHC-LC), and CHC-with HCC (CHC-HCC). Twenty-seven healthy individuals also participated as controls. Flow cytometry was used to analyze CD19<jats:sup>+<\/jats:sup> B cell subsets based on the expression of CD24 and CD38. CD19<jats:sup>+<\/jats:sup>CD24<jats:sup>hi<\/jats:sup>CD38<jats:sup>hi<\/jats:sup> Immature\/transitional B cells elevated in diabetic than non-diabetic patients. In diabetic patients, while CD19<jats:sup>+<\/jats:sup>CD24<jats:sup>+<\/jats:sup>CD38<jats:sup>\u2212<\/jats:sup> primarily memory B cells were higher in CHC-N and CHC-HCC groups than LC with a good predictive accuracy of LC, the opposite was observed for CD19<jats:sup>+<\/jats:sup>CD24<jats:sup>\u2212<\/jats:sup>CD38<jats:sup>\u2212<\/jats:sup> new memory B cells. Only in diabetic patients, the CD19<jats:sup>+<\/jats:sup>CD24<jats:sup>int<\/jats:sup>CD38<jats:sup>int<\/jats:sup> na\u00efve mature B cells were high in CHC-HCC patients with good prognostic accuracy of HCC. Merely in diabetic patients, several correlations were observed between B cell subsets and liver function. Immature\/transitional B cells increase remarkably in diabetic CHCpatients and might have a role in liver disease progression. Memory and Na\u00efve B cells are good potential predictors of LC and HCCin diabetic CHCpatients, respectively. Further studies are needed to investigate the role of the CD19<jats:sup>+<\/jats:sup>CD24<jats:sup>\u2212<\/jats:sup>CD38<jats:sup>\u2212<\/jats:sup> new memory B cells in disease progression in CHC patients.<\/jats:p>","DOI":"10.1038\/s41598-020-77416-0","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T11:14:02Z","timestamp":1606130042000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Impact of deranged B cell subsets distribution in the development of HCV-related cirrhosis and HCC in type two diabetes mellitus"],"prefix":"10.1038","volume":"10","author":[{"given":"Fadwa A.","family":"Abdelwahab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled M.","family":"Hassanein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8541-7304","authenticated-orcid":false,"given":"Helal F.","family":"Hetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed O.","family":"Abdelmalek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa M.","family":"Zahran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-711X","authenticated-orcid":false,"given":"Omnia","family":"El-Badawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,23]]},"reference":[{"key":"77416_CR1","doi-asserted-by":"publisher","first-page":"S3","DOI":"10.1016\/j.jhep.2014.06.031","volume":"61","author":"J Dubuisson","year":"2014","unstructured":"Dubuisson, J. & Cosset, F. 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