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Gaud). This study aimed to reveal the metagenomic and metabolomic changes between the healthy- and obstacle-plant after a long period of continuous cropping. After 10\u00a0years of continuous cropping, ramie planted in some portions of the land exhibited weak growth and low yield (Obstacle-group), whereas, ramie planted in the other portion of the land grew healthy (Health-group). We collected rhizosphere soil and root samples from which measurements of soil chemical and plant physiochemical properties were taken. All samples were subjected to non-targeted gas chromatograph-mass spectrometer (GS\/MS) metabolome analysis. Further, metagenomics was performed to analyze the functional genes in rhizospheric soil organisms. Based on the findings, ramie in Obstacle-group were characterized by shorter plant height, smaller stem diameter, and lower fiber production than that in Health-group. Besides, the Obstacle-group showed a lower relative abundance of <jats:italic>Rhizobiaceae, Lysobacter antibioticus,<\/jats:italic> and <jats:italic>Bradyrhizobium japonicum<\/jats:italic>, but a higher relative abundance of <jats:italic>Azospirillum lipoferum<\/jats:italic> and <jats:italic>A. brasilense<\/jats:italic> compared to the Health-group. Metabolomic analysis results implicated cysteinylglycine (Cys-Gly), uracil, malonate, and glycerol as the key differential metabolites between the Health- and Obstacle-group. Notably, this work revealed that bacteria such as <jats:italic>Rhizobia<\/jats:italic> potentially synthesize IAA and are likely to reduce the biotic stress of ramie. <jats:italic>L. antibioticus<\/jats:italic> also exerts a positive effect on plants in the fight against biotic stress and is mediated by metabolites including orthophosphate, uracil, and Cys-Gly, which may serve as markers for disease risk. These bacterial effects can play a key role in plant resistance to biotic stress via metabolic and methionine metabolism pathways.<\/jats:p>","DOI":"10.1038\/s41598-020-77475-3","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T11:14:02Z","timestamp":1606130042000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of the rhizospheric microbe and metabolites that led by the continuous cropping of ramie (Boehmeria nivea L. 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