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A total of 179 patients undergoing laparoscopic ovarian cystectomy with stripping were randomly allocated into three groups: the bipolar coagulation group, the Epi-pledget group, and the coagulation after Epi-pledget (Epi &amp; Coagulation) group. Serum anti-M\u00fcllerian hormone (AMH) levels and antral follicle count (AFC) by ultrasonography were measured to determine the preservation of ovarian function. To evaluate the postoperative ovarian cellular proliferative activity and tissue damage in a mouse model, we operated on the ovaries of mice with an artificial incision injury and applied two hemostatic methods: coagulation and Epi-pledget. Eight weeks after surgery, the AMH rate significantly decreased in the bipolar coagulation group compared with the Epi-pledget group. The AFC decline rate was also significantly greater in the coagulation group than the Epi-pledget group. Specifically, patients with endometrioma had a significantly greater decline of serum AMH in the coagulation group than the Epi-pledget group. In a histopathological analysis in mice, the Epi-pledget group showed ameliorated fibrotic changes and necrotic findings in the injured lesion compared with the bipolar coagulation group. The Epi-pledget method for ovarian stripping has an additional benefit of maximizing the preservation of the ovarian reserve, especially for the endometriotic ovarian cyst type.<\/jats:p>","DOI":"10.1038\/s41598-020-77781-w","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T11:05:07Z","timestamp":1606820707000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Epinephrine minimizes the use of bipolar coagulation and preserves ovarian reserve in laparoscopic ovarian cystectomy: a randomized controlled trial"],"prefix":"10.1038","volume":"10","author":[{"given":"Eun Young","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu-Hee","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"San-Hui","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu-Sang","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Jin","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Kuy","family":"Cha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"77781_CR1","doi-asserted-by":"publisher","first-page":"2589","DOI":"10.1016\/j.fertnstert.2011.01.036","volume":"95","author":"M Kitajima","year":"2011","unstructured":"Kitajima, M. et al. Changes in serum anti-M\u00fcllerian hormone levels may predict damage to residual normal ovarian tissue after laparoscopic surgery for women with ovarian endometrioma. Fertil. Steril. 95, 2589-2591.e2581. https:\/\/doi.org\/10.1016\/j.fertnstert.2011.01.036 (2011).","journal-title":"Fertil. Steril."},{"key":"77781_CR2","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.ajog.2006.01.078","volume":"195","author":"M Busacca","year":"2006","unstructured":"Busacca, M. et al. Determinants of long-term clinically detected recurrence rates of deep, ovarian, and pelvic endometriosis. Am. J. Obstet. Gynecol. 195, 426\u2013432. https:\/\/doi.org\/10.1016\/j.ajog.2006.01.078 (2006).","journal-title":"Am. J. Obstet. Gynecol."},{"key":"77781_CR3","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1093\/humrep\/dev313","volume":"31","author":"L Muzii","year":"2016","unstructured":"Muzii, L. et al. 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