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Thus, to confirm this finding, supportive functional analyses were performed on <jats:italic>Shroom3<\/jats:italic> in mice using fullerene-based siRNA delivery, which demonstrated that <jats:italic>Shroom3<\/jats:italic> knockdown led to albuminuria and podocyte foot process effacement. The in vitro experiment shows that knockdown of <jats:italic>Shroom3<\/jats:italic> caused defective formation of lamellipodia in podocyte, which would lead to the disruption of slit diaphragm. 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