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To detect <jats:italic>H. pylori,<\/jats:italic> a nanohybrid-based BabA immunosensor is developed herein. BabA is an outer membrane protein and one of the major virulence factors of <jats:italic>H. pylori<\/jats:italic>. To design the immunosensor, an Au electrode is loaded with palladium nanoparticles\u00a0(Pd<jats:sub>nano<\/jats:sub>) by electrodeposition to generate\u00a0reduced graphene oxide (rGO)\/poly(3,4-ethylenedioxythiophene) (PEDOT). The immobilization of these nanostructured materials imparts a large surface area and electroconductivity to bio-immune-sensing molecules (here, the BabA antigen and antibodies). After optimization, the fabricated immunosensor has the ability to detect antigens <jats:italic>(H. pylori)<\/jats:italic> in a linear range from 0.2 to 20\u00a0ng\/mL with a low LOD (0.2\u00a0ng\/mL). The developed immunosensor is highly specific, sensitive and reproducible. Additionally, in silico methods were employed to better understand the hybrid nanomaterials of the fabricated Pd<jats:sub>nano<\/jats:sub>\/rGO\/PEDOT\/Au electrode. Simulations performed by molecular docking, and Metropolis Monte Carlo adsorption studies were conducted. The results revealed that the hybrid nanomaterials exhibit a stable antigen\u2013antibody complex of BabA, yielding the lowest binding energy in relation to the electrode materials, emphasizing the functionality of the constructed electrodes in the electrochemical immunosensor.<\/jats:p>","DOI":"10.1038\/s41598-020-78068-w","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T11:06:08Z","timestamp":1607079968000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Nanohybrid-based immunosensor prepared for Helicobacter pylori BabA antigen detection through immobilized antibody assembly with @ Pdnano\/rGO\/PEDOT sensing platform"],"prefix":"10.1038","volume":"10","author":[{"given":"Shaivya","family":"Gupta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utkarsh","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bayu Tri","family":"Murti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athika Darumas","family":"Putri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Chauhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"78068_CR1","doi-asserted-by":"publisher","first-page":"786","DOI":"10.3390\/molecules22050786","volume":"22","author":"JPL Damasceno","year":"2017","unstructured":"Damasceno, J. 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