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Low-intensity eccentric training (LIET) has been used as a rehabilitation method in skeletal muscles after disuse. Recently, LIET has also been used for rehabilitating dystrophic muscles, but its effects are still unclear. The purpose of this study was to investigate the effects of 21\u00a0days of LIET in dystrophic soleus muscle. Thirty-six male <jats:italic>mdx<\/jats:italic> mice were randomized into six groups (n\u2009=\u20096\/each): <jats:italic>mdx<\/jats:italic> sedentary group; <jats:italic>mdx<\/jats:italic> training group-3\u00a0days; <jats:italic>mdx<\/jats:italic> training group-21\u00a0days; wild-type sedentary group; wild-type training group-3\u00a0days and wild-type training group-21\u00a0days. After the training sessions, animals were euthanized, and fragments of soleus muscles were removed for immunofluorescence and histological analyses, and measurements of active force and Ca<jats:sup>2+<\/jats:sup> sensitivity of the contractile apparatus. Muscles of the <jats:italic>mdx<\/jats:italic> training group-21\u00a0days showed an improvement in morphological characteristics and an increase of active force when compared to the sedentary <jats:italic>mdx<\/jats:italic> group. The results show that LIET can improve the functionality of dystrophic soleus muscle in mice.<\/jats:p>","DOI":"10.1038\/s41598-020-79168-3","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T11:05:48Z","timestamp":1613041548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice"],"prefix":"10.1038","volume":"11","author":[{"given":"Paulo S.","family":"Pedrazzani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana O. 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