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Nevertheless, pig farms using artificial insemination (AI) repeatedly experienced in recent year\u2019s fertility problems with dramatic consequences due to toxic compounds from plastic semen bags. In particular, bisphenol A diglycidyl-ether (BADGE) present in multilayer plastic bags can leach into the semen and could affect the functionality of the spermatozoa. Former studies could not find any alterations in spermatozoa based on the exposure to BADGE. The aim of the study was to evaluate effects of BADGE on boar spermatozoa using an extended panel of spermatological methods. In spring 2019, a large drop in farrowing rates from 92.6\u2009\u00b1\u20092.3% to 63.7\u2009\u00b1\u200911.1% in four sow farms in Croatia was detected. In migration studies, BADGE could be identified as a causal toxic compound and leached into the extended semen in concentration of 0.37\u2009\u00b1\u20090.05\u00a0mg\/L. Detailed spermatological studies showed that significant predictors for effects on spermatozoa were different levels of motility and kinematic data after a prolonged storage time, thermo-resistance test (prolonged incubation time), mitochondrial activity, membrane integrity and fluidity. No serious effects were observed for sperm morphology and DNA fragmentation. These results provide new insights into the development of a new quality assurance concept for a detailed spermatological examination during testing of plastic materials for boar semen preservation. It could be shown that boar spermatozoa are an excellent biosensor to detect potential toxicity and fertility-relevant compounds.<\/jats:p>","DOI":"10.1038\/s41598-020-79415-7","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T11:04:23Z","timestamp":1608203063000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of a panel of spermatological methods for assessing reprotoxic compounds in multilayer semen plastic bags"],"prefix":"10.1038","volume":"10","author":[{"given":"M.","family":"Schulze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Schr\u00f6ter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Jakop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"79415_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0378-4320(00)00157-3","volume":"62","author":"LA Johnson","year":"2000","unstructured":"Johnson, L. 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