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Dried powder of <jats:italic>M. glyptostroboides<\/jats:italic> floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid\u2013liquid extraction using the organic solvents <jats:italic>n<\/jats:italic>-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50\u00a0\u00b5g\/ml of the DME extract. Further, the sub-G1 population was determined by FACS analysis. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. Our results suggest that the extracts of <jats:italic>M. glyptostroboides<\/jats:italic> floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC\u2013MS analysis, could a potential natural anticancer agent.<\/jats:p>","DOI":"10.1038\/s41598-020-79573-8","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T11:26:43Z","timestamp":1610537203000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway"],"prefix":"10.1038","volume":"11","author":[{"given":"Hoomin","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheolwoo","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suji","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debasish Kumar","family":"Dey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung Kyo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivek K.","family":"Bajpai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Kyu","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Suk","family":"Huh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,13]]},"reference":[{"key":"79573_CR1","doi-asserted-by":"publisher","first-page":"526","DOI":"10.4143\/crt.2016.316","volume":"49","author":"EK Moon","year":"2017","unstructured":"Moon, E. 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