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Insecticide-based mosquito control has been the primary method for mitigating transmission of arboviruses. The consequences for the application of insecticides include both lethal and sublethal effects, and associated development of insecticide resistance. However, little is known about the influence on arboviral transmission. Mosquitoes with phenotypes that exhibit insecticide resistance or experience sublethal effects may be associated with altered susceptibility to arbovirus infection and transmission. Juvenile hormone analogs (JHAs) are insecticides that prevent pupa to adult molting of mosquitoes by mimicking the action of their natural juvenile hormone. Here, we examined whether the JHA pyriproxyfen interacts with ambient temperature (20\u00a0\u00b0C and 30\u00a0\u00b0C) during juvenile stages to influence life-history traits, population growth (\u03bb'), and Zika virus (ZIKV) infection in <jats:italic>Aedes aegypti<\/jats:italic>. Development time of females was lengthened at 20\u00a0\u00b0C and in the presence of JHA. Prevention of pupa to adult molting by JHA was differentially higher at elevated temperature than low temperature. Size of females was larger at 20\u00a0\u00b0C and smaller at 30\u00a0\u00b0C. Infection, disseminated infection, and transmission of ZIKV in females were enhanced by JHA at both 20\u00a0\u00b0C and 30\u00a0\u00b0C relative to the controls. These results demonstrate that mosquito life-history and vector competence parameters are strongly influenced by interactive effects of JHA and temperature. The JHA-induced enhancement of ZIKV infection in females should be a consideration when implementing JHA in vector control strategies.<\/jats:p>","DOI":"10.1038\/s41598-021-00432-1","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T10:02:49Z","timestamp":1635242569000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Juvenile hormone analog enhances Zika virus infection in Aedes aegypti"],"prefix":"10.1038","volume":"11","author":[{"given":"Abdullah A.","family":"Alomar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley H.","family":"Eastmond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry W.","family":"Alto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"432_CR1","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1146\/annurev.ento.45.1.371","volume":"45","author":"J Hemingway","year":"2000","unstructured":"Hemingway, J. & Ranson, H. 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