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Our objective in this study, therefore, was to determine the seroprevalence and to identify the risk factors associated with WNV infection in the most densely horse-populated governorates in Egypt. A cross-sectional study was conducted in 2018 on 930 horses, which were distributed over five governorates in the Nile delta of Egypt. The horses, which were randomly selected, were serologically tested through use of an ID screen West Nile competition enzyme-linked immunosorbent assay (ELISA) to detect anti-WNV immunoglobulin G (IgG) and plaque reduction neutralization tests (PRNT; gold standard) to confirm the seropositive status of animals and to avoid cross reaction with other flavi-viruses. Four variables (geographical location, breed, sex and age) were considered in the risk analysis. Univariable and stepwise forward multivariable logistic regression methods were used for risk-factor analysis. The odds ratio (OR) was used as an approximate measure of relative risk. A total of 156 (16.8%; 95% confidence interval (CI) 14.4\u201319.2; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) serum samples were found to be serologically positive for WNV. The highest seroprevalence rate was detected in horses of age \u2265\u200915\u00a0years (68.1%; 95% CI 49.8\u201372.4), stallions (26.4%; 95% CI 22.7\u201330.4), and those of mixed breed (21.5%; 95% CI 17.7\u201327.5). Horses older than 15\u00a0years were found to be at increased risk of WNV infection with OR\u2009=\u20094.3 (95% CI 3.0\u20136.2, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) compared with horses aged under 2.5\u00a0years. Also, when all the risk factors were considered, stallions were more likely than mares to be WNV seropositive (OR\u2009=\u20092.4, 95% CI 1.6\u20133.7, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), and of the breeds, mixed-breed (OR\u2009=\u20091.9, 95% CI 1.2\u20132.8, <jats:italic>P<\/jats:italic>\u2009=\u20090.005) and Arabian horses (OR\u2009=\u20091.9, 95% CI 1.2\u20132.8, <jats:italic>P<\/jats:italic>\u2009=\u20090.005) were more likely to be seropositive. Geographical location seemed to have no impact on the seroprevalence of exposure to WNV among these horses. Due to these findings, we strongly recommend intensive surveillance and implementation of effective control and prevention strategies against WNV, especially in stallion, mixed-breed horses with ages \u2265\u200915\u00a0years.<\/jats:p>","DOI":"10.1038\/s41598-021-00449-6","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T10:13:12Z","timestamp":1634897592000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["West Nile virus seroprevalence and associated risk factors among horses in Egypt"],"prefix":"10.1038","volume":"11","author":[{"given":"Abdelfattah","family":"Selim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameer","family":"Megahed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Kandeel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Alouffi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashal M.","family":"Almutairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"key":"449_CR1","doi-asserted-by":"publisher","first-page":"e153","DOI":"10.1111\/tbed.12341","volume":"63","author":"A Benjelloun","year":"2016","unstructured":"Benjelloun, A., El Harrak, M. & Belkadi, B. 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