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In a well-established polymerase switch model, Pol\u03b7 is thought to be a preferred TLS polymerase to insert correct nucleotides across from the thymine dimer, and Rev1 plays a scaffold role through physical interaction with Pol\u03b7 and the Rev7 subunit of Pol\u03b6 for continual DNA synthesis. Defective Pol\u03b7 causes a variant form of xeroderma pigmentosum (XPV), a disease with predisposition to sunlight-induced skin cancer. Previous studies revealed that expression of Rev1 alone is sufficient to confer enhanced UV damage tolerance in mammalian cells, which depends on its physical interaction with Pol\u03b6 but is independent of Pol\u03b7, a conclusion that appears to contradict current literature on the critical roles of Pol\u03b7 in TLS. To test a hypothesis that the Rev1 catalytic activity is required to backup Pol\u03b7 in TLS, we found that the Rev1 polymerase-dead mutation is synergistic with either Pol\u03b7 mutation or the Pol\u03b7-interaction mutation in response to UV-induced DNA damage. On the other hand, functional complementation of <jats:italic>polH<\/jats:italic> cells by Pol\u03b7 relies on its physical interaction with Rev1. Hence, our studies reveal critical interactions between Rev1 and Pol\u03b7 in response to UV damage.<\/jats:p>","DOI":"10.1038\/s41598-021-00878-3","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T11:04:13Z","timestamp":1635764653000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genetic and physical interactions between Pol\u03b7 and Rev1 in response to UV-induced DNA damage in mammalian cells"],"prefix":"10.1038","volume":"11","author":[{"given":"Tonghui","family":"Bi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunping","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,1]]},"reference":[{"key":"878_CR1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/emboj.2008.281","volume":"28","author":"S Shachar","year":"2009","unstructured":"Shachar, S. et al. 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