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Heterozygous gain of function mutations in <jats:italic>KCNJ11<\/jats:italic> can cause neonatal diabetes mellitus (NDM). In addition, many patients exhibit neurological defects ranging from modest learning disorders to severe cognitive dysfunction and seizures. However, it remains unclear to what extent these neurological deficits are due to direct brain-specific activity of mutant K<jats:sub>ATP<\/jats:sub>. We have generated cerebral organoids derived from human induced pluripotent stem cells (hiPSCs) possessing the <jats:italic>KCNJ11<\/jats:italic> mutation p.Val59Met (V59M) and from non-pathogenic\/normal hiPSCs (i.e., control\/WT). Control cerebral organoids developed neural networks that could generate stable synchronized bursting neuronal activity whereas those derived from V59M cerebral organoids showed reduced synchronization. Histocytochemical studies revealed a marked reduction in neurons localized to upper cortical layer-like structures in V59M cerebral organoids suggesting dysfunction in the development of cortical neuronal network. Examination of temporal transcriptional profiles of neural stem cell markers revealed an extended window of SOX2 expression in V59M cerebral organoids. Continuous treatment of V59M cerebral organoids with the K<jats:sub>ATP<\/jats:sub> blocker tolbutamide partially rescued the neurodevelopmental differences. Our study demonstrates the utility of human cerebral organoids as an investigative platform for studying the effects of KCNJ11 mutations on neurophysiological outcome.<\/jats:p>","DOI":"10.1038\/s41598-021-00939-7","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T11:04:18Z","timestamp":1635937458000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Developmental defects and impaired network excitability in a cerebral organoid\u00a0model of\u00a0KCNJ11 p.V59M-related neonatal diabetes"],"prefix":"10.1038","volume":"11","author":[{"given":"Gokhan","family":"Dalgin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew K.","family":"Tryba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley P.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo-Young","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis H.","family":"Philipson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siri Atma W.","family":"Greeley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Alfredo J.","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"939_CR1","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1002\/humu.23995","volume":"41","author":"E De Franco","year":"2020","unstructured":"De Franco, E. et al. 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