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In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prevent infarct progression, cerebral thrombosis as cause of secondary infarct growth has remained a matter of debate. As cerebral thrombi are frequently observed after experimental stroke, a thrombus-induced impairment of the brain microcirculation is considered to contribute to tissue damage. Here, we combine the model of transient middle cerebral artery occlusion (tMCAO) with light sheet fluorescence microscopy and immunohistochemistry of brain slices to investigate the kinetics of thrombus formation and infarct progression. Our data reveal that tissue damage already peaks after 8\u00a0h of reperfusion following 60\u00a0min MCAO, while cerebral thrombi are only observed at later time points. Thus, cerebral thrombosis is not causative for secondary infarct growth during ischemic stroke.<\/jats:p>","DOI":"10.1038\/s41598-021-02360-6","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T06:02:33Z","timestamp":1637733753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Infarct growth precedes cerebral thrombosis following experimental stroke in mice"],"prefix":"10.1038","volume":"11","author":[{"given":"Vanessa","family":"G\u00f6b","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian G.","family":"Voll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherina","family":"Hemmen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Stoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Nieswandt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Schuhmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin G.","family":"Heinze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Stegner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"key":"2360_CR1","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1161\/01.str.19.9.1083","volume":"19","author":"J Bogousslavsky","year":"1988","unstructured":"Bogousslavsky, J., Van Melle, G. & Regli, F. The Lausanne Stroke Registry: analysis of 1000 consecutive patients with first stroke. Stroke 19, 1083\u20131092. https:\/\/doi.org\/10.1161\/01.str.19.9.1083 (1988).","journal-title":"Stroke"},{"key":"2360_CR2","doi-asserted-by":"publisher","first-page":"3445","DOI":"10.2147\/DDDT.S56071","volume":"9","author":"F Fluri","year":"2015","unstructured":"Fluri, F., Schuhmann, M. K. & Kleinschnitz, C. Animal models of ischemic stroke and their application in clinical research. Drug Des. Devel. Ther. 9, 3445\u20133454. https:\/\/doi.org\/10.2147\/DDDT.S56071 (2015).","journal-title":"Drug Des. Devel. Ther."},{"key":"2360_CR3","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1038\/jcbfm.2010.66","volume":"30","author":"DW Howells","year":"2010","unstructured":"Howells, D. W. et al. Different strokes for different folks: the rich diversity of animal models of focal cerebral ischemia. J. Cereb. Blood Flow Metab. 30, 1412\u20131431. https:\/\/doi.org\/10.1038\/jcbfm.2010.66 (2010).","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"2360_CR4","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1161\/CIRCULATIONAHA.115.016371","volume":"132","author":"Z Zhu","year":"2015","unstructured":"Zhu, Z. et al. Combination of the immune modulator fingolimod with alteplase in acute ischemic stroke: a pilot trial. Circulation 132, 1104\u20131112. https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.115.016371 (2015).","journal-title":"Circulation"},{"key":"2360_CR5","doi-asserted-by":"publisher","first-page":"719","DOI":"10.3389\/fneur.2019.00719","volume":"10","author":"Z Zhang","year":"2019","unstructured":"Zhang, Z., Pu, Y., Mi, D. & Liu, L. Cerebral hemodynamic evaluation after cerebral recanalization therapy for acute ischemic stroke. Front. Neurol. 10, 719. https:\/\/doi.org\/10.3389\/fneur.2019.00719 (2019).","journal-title":"Front. 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