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The results indicated that both strains of mice when infected with HN878 exhibited significant protection in their lungs with BCG doses at 3\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u20143000\u00a0CFU (BALB\/c) and 3\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2014300\u00a0CFU (CB6F1). Whereas, a significant protection was seen in both strains of mice with BCG doses at 3\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2014300\u00a0CFU when infected with H37Rv. A significant increase in the frequencies of BCG-specific IFN\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    IL2\n                    <jats:sup>+<\/jats:sup>\n                    TNF\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    CD4 T cells in the BCG doses at 3\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u20143000\u00a0CFU (BALB\/c) and 3\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2014300\u00a0CFU (CB6F1) was seen. The IFN\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    IL2\n                    <jats:sup>+<\/jats:sup>\n                    TNF\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    CD4 T cells correlated with the\n                    <jats:italic>Mtb<\/jats:italic>\n                    burden in the lungs of HN878 infected mice (BALB\/c and CB6F1) whereas, IFN\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    TNF\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    CD4 T cells correlated with the BALB\/c mice infected with H37Rv or HN878. The BCG dose at 3000\u00a0CFU (an equivalent single human dose in the mice by body weight) is protective in both strains of mice infected with H37Rv or HN878 and may serve an interesting dose to test new TB vaccine in a preclinical animal model.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-02442-5","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T06:03:04Z","timestamp":1638424984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Efficacy and immunogenicity of different BCG doses in BALB\/c and CB6F1 mice when challenged with H37Rv or Beijing HN878"],"prefix":"10.1038","volume":"11","author":[{"given":"Bhagwati","family":"Khatri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Keeble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda","family":"Dagg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryan A.","family":"Kaveh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Hogarth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei Mei","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,2]]},"reference":[{"key":"2442_CR1","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1016\/S0140-6736(95)92348-9","volume":"346","author":"PE Fine","year":"1995","unstructured":"Fine, P. 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