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However, multiple SSA countries have reported <jats:italic>pfhrp2<\/jats:italic> and <jats:italic>pfhrp3<\/jats:italic> (<jats:italic>pfhrp2\/3<\/jats:italic>) gene deletions. Blood samples (n\u2009=\u20091109) collected from patients with <jats:italic>P. falciparum<\/jats:italic> infection from six health facilities throughout the Democratic Republic of the Congo (DRC) from March 2017 to January 2018 were evaluated for <jats:italic>pfhrp<\/jats:italic>2\/3 deletions. Samples were assayed for HRP2, pan-<jats:italic>Plasmodium<\/jats:italic> LDH (pLDH) and aldolase (pAldolase) antigens by bead-based multiplex antigen assay. Samples with low HRP2 concentration compared to pLDH and pAldolase antigens were selected for further <jats:italic>pfhrp2\/3<\/jats:italic> genotyping PCRs. The majority of blood samples (93.3%, 1035\/1109) had high concentrations of the HRP2 antigen. Single deletions of <jats:italic>pfhrp2<\/jats:italic> were identified in 0.27% (3\/1109) of screened samples, with one sample from each of the Kapolowe, Mikalayi, and Rutshuru study sites. A <jats:italic>pfhrp3<\/jats:italic> single deletion (0.09%, 1\/1109) was found in the Kapolowe site. Dual <jats:italic>pfhrp2<\/jats:italic> and <jats:italic>pfhrp3<\/jats:italic> deletions were not observed. Due to, the low numbers of <jats:italic>pfhrp2<\/jats:italic> deletions and the sporadic locations of these deletions, the use of HRP2-based RDTs appears to still be appropriate for these locations in DRC.<\/jats:p>","DOI":"10.1038\/s41598-021-02452-3","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T11:02:47Z","timestamp":1637924567000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018"],"prefix":"10.1038","volume":"11","author":[{"given":"Jessica N.","family":"McCaffery","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Nace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camelia","family":"Herman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balwan","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric Mukomena","family":"Sompwe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Papy Mandoko","family":"Nkoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieudonn\u00e9 Mumba","family":"Ngoyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gauthier Mesia","family":"Kahunu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Halsey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Rogier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,26]]},"reference":[{"key":"2452_CR1","unstructured":"WHO. 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