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Anti-nucleocapsid immunoglobulin G (anti-N IgG) titer in serum samples collected at a single (N\u2009=\u2009302) or multiple time points (N\u2009=\u2009229) 3\u201312\u00a0months after COVID-19 symptom onset or SARS-CoV-2 detection in respiratory specimens was measured by semiquantitative chemiluminescent microparticle immunoassay. The 531 patients (966 specimens) were classified according to the presence or absence of pneumonia symptoms. Anti N IgG was detected in 87.5% of patients (328\/375) at 3\u00a0months, 38.6% (93\/241) at 6\u00a0months, 23.7% (49\/207) at 9\u00a0months, and 26.6% (38\/143) at 12\u00a0months. The anti-N IgG seropositivity rate was significantly lower at 6, 9, and 12\u00a0months than at 3\u00a0months (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01) and was higher in the pneumonia group than in the non-pneumonia\/asymptomatic group at 6\u00a0months (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01), 9\u00a0months (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.04), and 12\u00a0months (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.04). The rate started to decline 6\u201312\u00a0months after symptom onset. Anti-N IgG sample\/cutoff index was positively correlated with age (r\u2009=\u20090.192,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01) but negatively correlated with interval between symptom onset and blood sampling (r\u2009=\u2009\u2009\u2212\u20090.567,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01). These findings can guide vaccine strategies in recovered COVID-19 patients.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-02659-4","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T06:04:42Z","timestamp":1638338682000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Long-term specific IgG response to SARS-CoV-2 nucleocapsid protein in recovered COVID-19 patients"],"prefix":"10.1038","volume":"11","author":[{"given":"Jira","family":"Chansaenroj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritthideach","family":"Yorsaeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawarat","family":"Posuwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiratchaya","family":"Puenpa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasamon","family":"Wanlapakorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natthinee","family":"Sudhinaraset","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manit","family":"Sripramote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piti","family":"Chalongviriyalert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supunee","family":"Jirajariyavej","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phatharaporn","family":"Kiatpanabhikul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jatuporn","family":"Saiyarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulikorn","family":"Soudon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orawan","family":"Thienfaidee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thitisan","family":"Palakawong Na Ayuthaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantapat","family":"Brukesawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chintana","family":"Chirathaworn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duangnapa","family":"Intharasongkroh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dootchai","family":"Chaiwanichsiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mila","family":"Issarasongkhram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungrueng","family":"Kitphati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anek","family":"Mungaomklang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pijaya","family":"Nagavajara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Poovorawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,1]]},"reference":[{"key":"2659_CR1","doi-asserted-by":"publisher","first-page":"1708","DOI":"10.1056\/NEJMoa2002032","volume":"382","author":"WJ Guan","year":"2020","unstructured":"Guan, W. 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