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This study aimed to assess the effect of the commercial early weaning on gut microbiota, intestinal gene expression and serum metabolomic response via an integrated-omic approach combining 16S rRNA gene sequencing, the OpenArray gene expression technology and\n                    <jats:sup>1<\/jats:sup>\n                    H-NMR spectroscopy. Fourteen piglets from different litters were sampled for blood, jejunum tissue and caecal content two days before (\u2212\u00a02d), and three days after (+\u20093d) weaning. A clearly differential ordination of caecal microbiota was observed. Higher abundances of\n                    <jats:italic>Roseburia<\/jats:italic>\n                    ,\n                    <jats:italic>Ruminococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Coprococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Dorea<\/jats:italic>\n                    and\n                    <jats:italic>Lachnospira<\/jats:italic>\n                    genera in weaned piglets compared to prior to weaning showed the quick microbial changes of the piglets\u2019 gut microbiota. Downregulation of\n                    <jats:italic>OCLN<\/jats:italic>\n                    ,\n                    <jats:italic>CLDN4<\/jats:italic>\n                    ,\n                    <jats:italic>MUC2<\/jats:italic>\n                    ,\n                    <jats:italic>MUC13<\/jats:italic>\n                    ,\n                    <jats:italic>SLC15A1<\/jats:italic>\n                    and\n                    <jats:italic>SLC13A1<\/jats:italic>\n                    genes, also evidenced the negative impact of weaning on gut barrier and digestive functions. Metabolomic approach pinpointed significant decreases in choline, LDL, triglycerides, fatty acids, alanine and isoleucine and increases in 3-hydroxybutyrate after weaning. Moreover, the correlation between microbiota and metabolome datasets revealed the existence of metabolic clusters interrelated to different bacterial clusters. Our results demonstrate the impact of weaning stress on the piglet and give insights regarding the associations between gut microbiota and the animal gene activity and metabolic response.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-02754-6","type":"journal-article","created":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T06:06:05Z","timestamp":1638770765000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Understanding host-microbiota interactions in the commercial piglet around weaning"],"prefix":"10.1038","volume":"11","author":[{"given":"M.","family":"Saladrigas-Garc\u00eda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"D\u2019Angelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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