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Cases of acute renal failure in humans caused by<jats:italic>Babesia<\/jats:italic>spp. have been described in the literature. The kidneys are characterised by intense blood flow through the blood vessels, which increases the likelihood of contact with the intra-erythrocyte parasite. The aim of this study was to observe the influence of<jats:italic>B. microti<\/jats:italic>(ATCC 30221) on renal epithelial cells in vitro cultured (NRK-52E line) and Wistar rats\u2019 kidney. Both NRK-52E cells and rats\u2019 kidney sections were analysed by light microscopy, transmission electron microscopy (TEM) and fluorescence in situ hybridization (FISH). Necrotic changes in renal epithelial cells have been observed in vitro and in vivo. In many cross-sections through the rats\u2019 kidney, adhesion of blood cells to the vascular endothelium, accumulation of erythrocytes and emboli were demonstrated. In NRK-52E culture, elements with a distinctly doubled cell membrane resembling<jats:italic>B. microti<\/jats:italic>were found inside the cytoplasm and adjacent to the cell layer. The study indicates a chemotactic tendency for<jats:italic>B. microti<\/jats:italic>to adhere to the renal tubules' epithelium, a possibility of piroplasms entering the renal epithelial cells, their proliferation within the cytoplasm and emboli formation.<\/jats:p>","DOI":"10.1038\/s41598-021-03079-0","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T11:03:07Z","timestamp":1638961387000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model"],"prefix":"10.1038","volume":"11","author":[{"given":"Marta","family":"Alberty\u0144ska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Ok\u0142a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Jasik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danuta","family":"Urba\u0144ska-Jasik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Przemys\u0142aw","family":"Pol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"issue":"5","key":"3079_CR1","doi-asserted-by":"publisher","first-page":"487","DOI":"10.14202\/vetworld.2016.487-495","volume":"9","author":"BR Maharana","year":"2016","unstructured":"Maharana, B. 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