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Three practical diets with graded Met levels (0.40%, 0.84% and 1.28% dry matter) were prepared to feed the juvenile fish. The results showed that the 0.84% Met diet significantly improved the growth compared with 0.40% diets. Compared with 0.84% and 1.28% Met, 0.40% Met significantly increased the hepatic lipid content, while decreasing the muscular lipid and glycogen contents.\u00a00.40% Met decreased the protein levels of phospho-Eukaryotic initiation factor 4E binding protein-1 (p-4e-bp1), 4e-bp1 and Ribosomal protein S6 kinase 1 in the liver, compared with 0.84% diet, while an increasing trend was observed in the\u00a0muscle. Met supplementation tended to decrease and increase lipid synthesis in the liver and muscle, respectively, via changing mRNA levels of\n                    <jats:italic>sterol regulatory element-binding protein 1<\/jats:italic>\n                    ,\n                    <jats:italic>fatty acid synthetase<\/jats:italic>\n                    and\n                    <jats:italic>acetyl-CoA carboxylase<\/jats:italic>\n                    . 1.28% dietary Met promoted fatty acid \u03b2-oxidation and lipolysis in both the liver and muscle by increasing\n                    <jats:italic>carnitine palmitoyl transferase 1<\/jats:italic>\n                    ,\n                    <jats:italic>peroxisome proliferator activated receptor alpha<\/jats:italic>\n                    ,\n                    <jats:italic>lipoprotein lipase<\/jats:italic>\n                    and\n                    <jats:italic>lipase<\/jats:italic>\n                    mRNA levels. Compared with 0.40% and 0.84% dietary Met, 1.28% Met enhanced the mRNA levels of hepatic gluconeogenesis related genes\n                    <jats:italic>phosphoenolpyruvate carboxykinase<\/jats:italic>\n                    (\n                    <jats:italic>pepck<\/jats:italic>\n                    ), and\n                    <jats:italic>glucose-6-phosphatase<\/jats:italic>\n                    , and muscular glycolysis related genes\n                    <jats:italic>phosphofructokinase<\/jats:italic>\n                    (\n                    <jats:italic>pfk<\/jats:italic>\n                    ), and\n                    <jats:italic>pyruvate kinase<\/jats:italic>\n                    (\n                    <jats:italic>pk<\/jats:italic>\n                    ). The mRNA levels of hepatic\n                    <jats:italic>pfk<\/jats:italic>\n                    ,\n                    <jats:italic>pk<\/jats:italic>\n                    and\n                    <jats:italic>glucokinase<\/jats:italic>\n                    were markedly downregulated by 1.28% Met compared with 0.84% level. Muscular\n                    <jats:italic>pepck<\/jats:italic>\n                    ,\n                    <jats:italic>glycogen synthase<\/jats:italic>\n                    , and hepatic\n                    <jats:italic>glucose transporters 2<\/jats:italic>\n                    mRNA levels were induced by 1.28% Met. Generally, deficient Met level decreased the growth of juvenile\n                    <jats:italic>Megalobrama amblycephala<\/jats:italic>\n                    , and the different nutrient metabolism responses to dietary Met were revealed in the liver and muscle.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-03084-3","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T06:04:31Z","timestamp":1639375471000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels"],"prefix":"10.1038","volume":"11","author":[{"given":"Ke","family":"Ji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualiang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingchun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianping","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangkun","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"issue":"6","key":"3084_CR1","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1111\/anu.12131","volume":"20","author":"YJ Liao","year":"2014","unstructured":"Liao, Y. 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