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The patients were divided into two groups: those with <jats:italic>Demodex<\/jats:italic> infestation and without visible symptoms of eyelid or eye surface diseases, and asymptomatic non-infested patients. The NIBUT analysis was performed with a 5\u00a0M keratograph (oculus). Non-invasive tests were performed to identify the first and mean values of the tear break up time. The first and mean tear break up time in the <jats:italic>Demodex<\/jats:italic>-infested group was lower than in the non-infested subjects. The difference was a highly statistically significant. There was a significant correlation with the age of the patients for the first break up time. The first break up time in both eyes decreased with the age of the <jats:italic>Demodex<\/jats:italic>-infested and non-infested patients. The NIBUT analyses indicate the impact of <jats:italic>Demodex<\/jats:italic> mites on the tear film stability. 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