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A total of 190 samples were collected from milk, farm personnel and different environmental components from the dairy farm under the study to isolate <jats:italic>S. aureus<\/jats:italic>. Cefotax based magnetic nanoparticles was synthetized by the adsorption method and marked using Fourier-transform infrared spectrum (FT-IR), and X-ray diffraction (XRD), then it was characterized using Scanning and Transmission Electron Microscope (SEM and TEM). The obtained results revealed that number of positive samples of <jats:italic>S. aureus<\/jats:italic> isolation were 63 (33.1%), mainly from feed manger followed by milk machine swabs (60.0 and 53.3%, respectively) at X<jats:sup>2<\/jats:sup>\u2009=\u200948.83 and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001. Obtained isolates were identified biochemically and by using molecular assays (PCR), also <jats:italic>mec<\/jats:italic> A gene responsible for resistance to cefotax was detected. Testing the sensitivity of 63 isolates of <jats:italic>S. aureus<\/jats:italic> showed variable degree of resistance to different tested antibiotics and significant sensitivity to cefotax based magnetic nanoparticles at <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05. It was concluded that dairy environment might act a potential source for transmission of MRSA between human and animal populations. 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