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Therefore, the AMR prevalence in shelter dogs is rarely known. Herein, we conducted a survey in animal shelters in Chiba and Kanagawa prefectures, in the Kanto Region, Japan, to ascertain the AMR status of <jats:italic>Escherichia coli<\/jats:italic> \u00a0(<jats:italic>E. coli<\/jats:italic>)\u00a0prevalent in shelter dogs. <jats:italic>E. coli<\/jats:italic> was detected in the fecal samples of all 61 and 77 shelter dogs tested in Chiba and Kanagawa, respectively. The AMR was tested against 20 antibiotics. <jats:italic>E. coli<\/jats:italic> isolates derived from 16.4% and 26.0% of samples from Chiba and Kanagawa exhibited resistance to at least one antibiotic, respectively. <jats:italic>E. coli<\/jats:italic> in samples from Chiba and Kanagawa prefectures were commonly resistant to ampicillin, piperacillin, streptomycin, kanamycin, tetracycline, and nalidixic acid; that from the Kanagawa Prefecture to cefazolin, cefotaxime, aztreonam, ciprofloxacin, and levofloxacin and that from Chiba Prefecture to chloramphenicol and imipenem. Multidrug-resistant bacteria were detected in 18 dogs from both regions; \u03b2-lactamase genes (<jats:italic>blaTEM<\/jats:italic>, <jats:italic>blaDHA-1<\/jats:italic>, <jats:italic>blaCTX-M-9<\/jats:italic> group <jats:italic>CTX-M-14<\/jats:italic>), quinolone-resistance protein genes (<jats:italic>qnrB<\/jats:italic> and <jats:italic>qnrS<\/jats:italic>), and mutations in quinolone-resistance-determining regions (<jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic>) were detected. These results could partially represent the AMR data in shelter dogs in the Kanto Region of Japan.<\/jats:p>","DOI":"10.1038\/s41598-021-04435-w","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T11:02:40Z","timestamp":1642158160000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Surveillance of antimicrobial-resistant Escherichia coli in Sheltered dogs in the Kanto Region of Japan"],"prefix":"10.1038","volume":"12","author":[{"given":"Akihisa","family":"Hata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Fujitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiko","family":"Ono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,14]]},"reference":[{"key":"4435_CR1","doi-asserted-by":"publisher","first-page":"1439","DOI":"10.3390\/microorganisms8091439","volume":"8","author":"JW Chen","year":"2020","unstructured":"Chen, J. 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