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A prospective diagnostic study was performed including 21 patients undergoing open knee surgery. HSI data were acquired during surgery, and the joint surface\u2019s cartilage was assessed according to the ICRS cartilage injury score. The HSI system records light spectra from 500 to 1000\u00a0nm and generates several parameters including tissue water index (TWI) and the absorbance at 960\u00a0nm and 540\u00a0nm. Receiver operating characteristic curves were calculated to assess test parameters for threshold values of HSI. Areas with a cartilage defect ICRS grade\u2009\u2265\u20093 showed a significantly lower TWI (<jats:italic>p<\/jats:italic>\u2009=\u20090.026) and higher values for 540\u00a0nm (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). No difference was seen for 960\u00a0nm (<jats:italic>p<\/jats:italic>\u2009=\u20090.244). For a threshold of 540\u00a0nm\u2009&gt;\u20090.74, a cartilage defect ICRS grade\u2009\u2265\u20093 could be detected with a sensitivity of 0.81 and a specificity of 0.81. TWI was not suitable for cartilage defect detection. HSI can provide reliable parameters to differentiate healthy and damaged cartilage. Our data clearly suggest that the difference in absorbance at 540\u00a0nm would be the best parameter to achieve accurate identification of damaged cartilage.<\/jats:p>","DOI":"10.1038\/s41598-021-04642-5","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T11:02:59Z","timestamp":1641985379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Intraoperative hyperspectral imaging (HSI) as a new diagnostic tool for the detection of cartilage degeneration"],"prefix":"10.1038","volume":"12","author":[{"given":"Max","family":"Kistler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannes","family":"K\u00f6hler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Theopold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Gockel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Hepp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Osterhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,12]]},"reference":[{"key":"4642_CR1","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1093\/bmb\/lds038","volume":"105","author":"A Litwic","year":"2013","unstructured":"Litwic, A., Edwards, M. 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