{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:50:12Z","timestamp":1792785012666,"version":"4.5.0"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T00:00:00Z","timestamp":1612310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T00:00:00Z","timestamp":1612310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"BMWF\/MINCyT Bilateral Cooperation Program","award":["AR06\/2013"],"award-info":[{"award-number":["AR06\/2013"]}]},{"name":"BMWF\/MINCyT Bilateral Cooperation Program","award":["AR06\/2013"],"award-info":[{"award-number":["AR06\/2013"]}]},{"name":"PUE","award":["0085-2016"],"award-info":[{"award-number":["0085-2016"]}]},{"name":"PUE","award":["0085-2016"],"award-info":[{"award-number":["0085-2016"]}]},{"DOI":"10.13039\/501100010253","name":"UBACyT","doi-asserted-by":"crossref","award":["20020170100397BA"],"award-info":[{"award-number":["20020170100397BA"]}],"id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010253","name":"UBACyT","doi-asserted-by":"crossref","award":["20020150100126BA"],"award-info":[{"award-number":["20020150100126BA"]}],"id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CONICET PIP","award":["1122015010031CO"],"award-info":[{"award-number":["1122015010031CO"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Salicylic acid (SAL) has recently been shown to induce biofilm formation in\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatory\n                    <jats:italic>agr<\/jats:italic>\n                    system and its impact on\n                    <jats:italic>S. aureus<\/jats:italic>\n                    biofilm formation. The\n                    <jats:italic>agr<\/jats:italic>\n                    quorum-sensing system, which is a central regulator in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    pathogenicity, plays a pivotal role in the dispersal of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    mature biofilms and contributes to the creation of new colonization sites. Here, we demonstrate that SAL impairs biofilm dispersal by interfering with\n                    <jats:italic>agr<\/jats:italic>\n                    expression. As revealed by our work, protease and surfactant molecule production is diminished, and bacterial cell autolysis is also negatively affected by SAL. Furthermore, as a consequence of SAL treatment, the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    biofilm matrix revealed the lack of extracellular DNA. In silico docking and simulation of molecular dynamics provided evidence for a potential interaction of AgrA and SAL, resulting in reduced activity of the\n                    <jats:italic>agr<\/jats:italic>\n                    system. In conclusion, SAL stabilized the mature\n                    <jats:italic>S. aureus<\/jats:italic>\n                    biofilms, which may prevent bacterial cell dissemination. However, it may foster the establishment of infections locally and consequently increase bacterial persistence leading to therapeutic failure.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-82308-y","type":"journal-article","created":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T06:10:36Z","timestamp":1612332636000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system"],"prefix":"10.1038","volume":"11","author":[{"given":"Cristian","family":"Dotto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Lombarte Serrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn","family":"Ledesma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Vay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Ehling-Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel O.","family":"Sordelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Grunert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Buzzola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,3]]},"reference":[{"key":"82308_CR1","first-page":"1","volume-title":"New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms. Ch 1","author":"MK Yadav","year":"2020","unstructured":"Yadav, M. K., Song, J. J., Singh, B. P. & Vidal, J. E. Microbial biofilms and human disease: a concise review. In New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms. Ch 1 (eds Yadav, M. K. & Singh, B. P.) 1\u201313 (Elsevier, Amsterdam, 2020)."},{"key":"82308_CR2","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1002\/bies.201400080","volume":"36","author":"BP Conlon","year":"2014","unstructured":"Conlon, B. P. Staphylococcus aureus chronic and relapsing infections: evidence of a role for persister cells: an investigation of persister cells, their formation and their role in S. aureus disease. BioEssays 36, 991\u2013996 (2014).","journal-title":"BioEssays"},{"key":"82308_CR3","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1016\/j.tim.2019.05.003","volume":"27","author":"A Crabb\u00e9","year":"2019","unstructured":"Crabb\u00e9, A., Jensen, P., Bjarnsholt, T. & Coenye, T. Antimicrobial tolerance and metabolic adaptations in microbial biofilms. Trends Microbiol. 27, 850\u2013863 (2019).","journal-title":"Trends Microbiol."},{"key":"82308_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1146\/annurev-med-042711-140023","volume":"64","author":"M Otto","year":"2013","unstructured":"Otto, M. Staphylococcal infections: mechanisms of biofilm maturation and detachment as critical determinants of pathogenicity. Annu. Rev. Med. 64, 1\u201314 (2013).","journal-title":"Annu. Rev. Med."},{"key":"82308_CR5","doi-asserted-by":"publisher","first-page":"5427","DOI":"10.1128\/IAI.67.10.5427-5433.1999","volume":"67","author":"SE Cramton","year":"1999","unstructured":"Cramton, S. E., Gerke, C., Schnell, N. F., Nichols, W. W. & G\u00f6tz, F. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect. Immun. 67, 5427\u20135433 (1999).","journal-title":"Infect. Immun."},{"key":"82308_CR6","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1111\/j.1574-6968.2007.00688.x","volume":"270","author":"JP O'Gara","year":"2007","unstructured":"O\u2019Gara, J. P. ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus. FEMS Microbiol. Lett. 270, 179\u2013188 (2007).","journal-title":"FEMS Microbiol. Lett."},{"key":"82308_CR7","doi-asserted-by":"publisher","first-page":"2888","DOI":"10.1128\/JB.183.9.2888-2896.2001","volume":"183","author":"C Cucarella","year":"2001","unstructured":"Cucarella, C., Solano, C. & Valle, J. Bap, a Staphylococcus aureus surface protein involved in biofilm formation. J. Bacteriol. 183, 2888\u20132896 (2001).","journal-title":"J. Bacteriol."},{"key":"82308_CR8","doi-asserted-by":"publisher","first-page":"8113","DOI":"10.1073\/pnas.0610226104","volume":"104","author":"K Rice","year":"2007","unstructured":"Rice, K. et al. The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proc. Natl. Acad. Sci. 104, 8113\u20138118 (2007).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"82308_CR9","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1073\/pnas.1115006109","volume":"109","author":"S Periasamy","year":"2012","unstructured":"Periasamy, S. et al. How Staphylococcus aureus biofilms develop their characteristic structure. Proc. Natl. Acad. Sci. 109, 1281\u20131286 (2012).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"82308_CR10","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1172\/JCI42520","volume":"121","author":"R Wang","year":"2011","unstructured":"Wang, R. et al. Staphylococcus epidermidis surfactant peptides promote biofilm maturation and dissemination of biofilm-associated infection in mice. J. Clin. Investig. 121, 238\u2013248 (2011).","journal-title":"J. Clin. Investig."},{"issue":"4","key":"82308_CR11","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1128\/microbiolspec.RWR-0017-2018","volume":"6","author":"S Svenningsen","year":"2018","unstructured":"Svenningsen, S. Small RNA-based regulation of bacterial quorum sensing and biofilm formation. Microbiol. Spectrum 6(4), 283\u2013304 (2018).","journal-title":"Microbiol. Spectrum"},{"key":"82308_CR12","doi-asserted-by":"crossref","unstructured":"Jenul, C. & Horswill, A. R. Regulation of Staphylococcus aureus virulence. In Gram\u2010Positive Pathogens, 669\u2013686 (2019).","DOI":"10.1128\/9781683670131.ch41"},{"key":"82308_CR13","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1146\/annurev-micro-102215-095708","volume":"70","author":"D Bronesky","year":"2016","unstructured":"Bronesky, D. et al. Staphylococcus aureus RNAIII and its regulon link quorum sensing, stress responses, metabolic adaptation, and regulation of virulence gene expression. Annu. Rev. Microbiol. 70, 299\u2013316 (2016).","journal-title":"Annu. Rev. Microbiol."},{"key":"82308_CR14","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.molcel.2008.08.005","volume":"32","author":"SY Queck","year":"2008","unstructured":"Queck, S. Y. et al. RNAIII-independent target gene control by the agr quorum sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus. Mol. Cell 32, 150\u2013158 (2008).","journal-title":"Mol. Cell"},{"issue":"4","key":"82308_CR15","doi-asserted-by":"publisher","first-page":"e1000052","DOI":"10.1371\/journal.ppat.1000052","volume":"4","author":"BR Boles","year":"2008","unstructured":"Boles, B. R. & Horswill, A. R. agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog. 4(4), e1000052 (2008).","journal-title":"PLoS Pathog."},{"key":"82308_CR16","doi-asserted-by":"publisher","first-page":"003685041989865","DOI":"10.1177\/0036850419898659","volume":"103","author":"Y Liu","year":"2020","unstructured":"Liu, Y., Zhang, J. & Ji, Y. Environmental factors modulate biofilm formation by Staphylococcus aureus. Sci. Prog. 103, 0036850419898659 (2020).","journal-title":"Sci. Prog."},{"key":"82308_CR17","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1016\/S0140-6736(18)31858-0","volume":"392","author":"P Vranckx","year":"2018","unstructured":"Vranckx, P. et al. Ticagrelor plus aspirin for 1 month, followed by ticagrelor monotherapy for 23 months vs aspirin plus clopidogrel or ticagrelor for 12 months, followed by aspirin monotherapy for 12 months after implantation of a drug-eluting stent: a multicentre, open-label, randomised superiority trial. Lancet 392, 940\u2013949 (2018).","journal-title":"Lancet"},{"key":"82308_CR18","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3389\/fmed.2019.00217","volume":"6","author":"N Hannachi","year":"2019","unstructured":"Hannachi, N., Habib, G. & Camoin-Jau, L. Aspirin effect on Staphylococcus aureus-platelet interactions during infectious endocarditis. Front. Med. 6, 217 (2019).","journal-title":"Front. Med."},{"key":"82308_CR19","first-page":"8:4","volume":"23","author":"CM Dotto","year":"2017","unstructured":"Dotto, C. M. et al. The active component of aspirin, salicylic acid, promotes Staphylococcus aureus biofilm formation in a PIA-dependent manner. Front. Microbiol. 23, 8:4 (2017).","journal-title":"Front. Microbiol."},{"key":"82308_CR20","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1016\/S1357-2725(00)00042-X","volume":"32","author":"CTD Price","year":"2000","unstructured":"Price, C. T. D., Lee, I. R. & Gustafson, J. E. The effects of salicylate on bacteria. Int. J. Biochem. Cell Biol. 32, 1029\u20131043 (2000).","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"82308_CR21","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.micpath.2014.07.008","volume":"74","author":"SA El-Mowafy","year":"2014","unstructured":"El-Mowafy, S. A., El Galil, K. H. A., El-Messery, S. M. & Shaaban, M. I. Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa. Microb. Pathog. 74, 25\u201332 (2014).","journal-title":"Microb. Pathog."},{"key":"82308_CR22","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1128\/JB.01276-10","volume":"193","author":"E Tsompanidou","year":"2011","unstructured":"Tsompanidou, E. et al. Requirement of the agr locus for colony spreading of Staphylococcus aureus. J. Bacteriol. 193, 1267\u20131272 (2011).","journal-title":"J. Bacteriol."},{"key":"82308_CR23","volume-title":"Encyclopedia of Analytical Chemistry","author":"D Naumann","year":"2006","unstructured":"Naumann, D. Infrared spectroscopy in microbiology. In Encyclopedia of Analytical Chemistry (ed. Meyers, R. A.) (Wiley, Hoboken, 2006)."},{"key":"82308_CR24","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.3389\/fmicb.2015.01174","volume":"6","author":"K Le","year":"2015","unstructured":"Le, K. & Otto, M. Quorum-sensing regulation in staphylococci\u2014an overview. Front. Microbiol. 6, 1174 (2015).","journal-title":"Front. Microbiol."},{"issue":"3","key":"82308_CR25","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1111\/j.1365-2958.2011.07720.x","volume":"81","author":"MA Benson","year":"2011","unstructured":"Benson, M. A. et al. Staphylococcus aureus regulates the expression and production of the staphylococcal superantigen-like secreted proteins in a Rot-dependent manner. Mol. Microbiol. 81(3), 659\u2013675 (2011).","journal-title":"Mol. Microbiol."},{"key":"82308_CR26","doi-asserted-by":"publisher","first-page":"3724","DOI":"10.1128\/JB.180.14.3724-3726.1998","volume":"180","author":"DF Fujimoto","year":"1998","unstructured":"Fujimoto, D. F. & Bayles, K. W. Opposing roles of the Staphylococcus aureus virulence regulators, agr and sar, in triton X-100- and penicillin-induced autolysis. J. Bacteriol. 180, 3724\u20133726 (1998).","journal-title":"J. Bacteriol."},{"key":"82308_CR27","doi-asserted-by":"publisher","first-page":"2547","DOI":"10.1002\/elan.201300342","volume":"25","author":"M Abedi","year":"2013","unstructured":"Abedi, M., Bathaie, S. Z. & Mousavi, M. F. Interaction between DNA and some salicylic acid derivatives and characterization of their DNA targets. Electroanalysis 25, 2547\u20132556 (2013).","journal-title":"Electroanalysis"},{"key":"82308_CR28","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","volume":"31","author":"O Trott","year":"2010","unstructured":"Trott, O. & Olson, A. J. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J. Comput. Chem. 31, 455\u2013461 (2010).","journal-title":"J. Comput. Chem."},{"key":"82308_CR29","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1002\/jcc.21256","volume":"16","author":"GM Morris","year":"2009","unstructured":"Morris, G. M. et al. AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility. J. Comput. Chem. 16, 2785\u20132791 (2009).","journal-title":"J. Comput. Chem."},{"key":"82308_CR30","doi-asserted-by":"publisher","first-page":"9095","DOI":"10.1073\/pnas.1200603109","volume":"109","author":"F Sun","year":"2012","unstructured":"Sun, F. et al. Quorum-sensing agr mediates bacterial oxidation response via an intramolecular disulfide redox switch in the response regulator AgrA. Proc. Natl. Acad. Sci. U.S.A. 109, 9095\u20139100 (2012).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"82308_CR31","doi-asserted-by":"publisher","first-page":"3738","DOI":"10.1128\/JB.186.12.3738-3748.2004","volume":"186","author":"J Gao","year":"2004","unstructured":"Gao, J. & Stewart, G. C. Regulatory elements of the Staphylococcus aureus protein A (Spa) promoter. J. Bacteriol. 186, 3738\u20133748 (2004).","journal-title":"J. Bacteriol."},{"key":"82308_CR32","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1161\/CIRCULATIONAHA.116.023952","volume":"134","author":"G Gargiulo","year":"2016","unstructured":"Gargiulo, G. et al. A critical appraisal of aspirin in secondary prevention: Is less more?. Circulation 134, 1881\u20131906 (2016).","journal-title":"Circulation"},{"key":"82308_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0147131","volume":"11","author":"AE Laudy","year":"2016","unstructured":"Laudy, A. E., Mrowka, A., Krajewska, J. & Tyski, S. The influence of efflux pump inhibitors on the activity of non-antibiotic NSAIDS against Gram-negative rods. PLoS ONE 11, 1\u201316 (2016).","journal-title":"PLoS ONE"},{"key":"82308_CR34","doi-asserted-by":"publisher","first-page":"105890","DOI":"10.1016\/j.ijantimicag.2020.105890","volume":"55","author":"N Hannachi","year":"2020","unstructured":"Hannachi, N. et al. Effect of antiplatelet agents on platelet anti-staphylococcal capacity, in vitro study. Int. J. Antimicrob. Agents 55, 105890 (2020).","journal-title":"Int. J. Antimicrob. Agents"},{"key":"82308_CR35","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1128\/JB.01149-06","volume":"189","author":"JT Riordan","year":"2007","unstructured":"Riordan, J. T. et al. Response of Staphylococcus aureus to salicylate challenge. J. Bacteriol. 189, 220\u2013227 (2007).","journal-title":"J. Bacteriol."},{"key":"82308_CR36","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1128\/IAI.00245-09","volume":"78","author":"LP Alvarez","year":"2010","unstructured":"Alvarez, L. P. et al. Salicylic acid diminishes Staphylococcus aureus capsular polysaccharide type 5 expression. Infect. Immun. 78, 1339\u20131344 (2010).","journal-title":"Infect. Immun."},{"key":"82308_CR37","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.micinf.2011.06.003","volume":"13","author":"LP Alvarez","year":"2011","unstructured":"Alvarez, L. P., Barbagelata, M. S., Cheung, A. L., Sordelli, D. O. & Buzzola, F. R. Salicylic acid enhances Staphylococcus aureus extracellular adhesin protein expression. Microbes Infect. 13, 1073\u20131080 (2011).","journal-title":"Microbes Infect."},{"key":"82308_CR38","doi-asserted-by":"publisher","first-page":"3521","DOI":"10.1007\/s00253-019-09618-0","volume":"103","author":"SA Ahmed","year":"2019","unstructured":"Ahmed, S. A. et al. Natural quorum sensing inhibitors effectively downregulate gene expression of Pseudomonas aeruginosa virulence factors. Appl. Microbiol. Biotechnol. 103, 3521\u20133535 (2019).","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"82308_CR39","doi-asserted-by":"publisher","first-page":"1688","DOI":"10.1086\/317606","volume":"182","author":"C Vuong","year":"2000","unstructured":"Vuong, C., Saenz, H. L., G\u00f6tz, F. & Otto, M. Impact of the agr quorum-sensing system on adherence to polystyrene in Staphylococcus aureus. J. Infect. Dis. 182, 1688\u20131693 (2000).","journal-title":"J. Infect. Dis."},{"key":"82308_CR40","doi-asserted-by":"publisher","first-page":"1838","DOI":"10.1128\/JB.186.6.1838-1850.2004","volume":"186","author":"JM Yarwood","year":"2004","unstructured":"Yarwood, J. M., Bartels, D. J., Volper, E. M. & Greenberg, E. P. Quorum sensing in Staphylococcus aureus biofilms. J. Bacteriol. 186, 1838\u20131850 (2004).","journal-title":"J. Bacteriol."},{"key":"82308_CR41","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.ijmm.2006.01.042","volume":"296","author":"KF Kong","year":"2006","unstructured":"Kong, K. F., Vuong, C. & Otto, M. Staphylococcus quorum sensing in biofilm formation and infection. Int. J. Med. Microbiol. 296, 133\u2013139 (2006).","journal-title":"Int. J. Med. Microbiol."},{"key":"82308_CR42","doi-asserted-by":"publisher","first-page":"2966","DOI":"10.1128\/IAI.00394-15","volume":"83","author":"S Dastgheyb","year":"2015","unstructured":"Dastgheyb, S. et al. Role of phenol-soluble modulins in formation of Staphylococcus aureus biofilms in synovial fluid. Infect. Immun. 83, 2966\u20132975 (2015).","journal-title":"Infect. Immun."},{"issue":"10","key":"82308_CR43","doi-asserted-by":"publisher","first-page":"e0164523","DOI":"10.1371\/journal.pone.0164523","volume":"11","author":"H Kizaki","year":"2016","unstructured":"Kizaki, H. et al. Cell-surface phenol soluble modulins regulate Staphylococcus aureus colony spreading. PLoS ONE 11(10), e0164523 (2016).","journal-title":"PLoS ONE"},{"key":"82308_CR44","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1021\/acscombsci.8b00141","volume":"21","author":"P Mester","year":"2019","unstructured":"Mester, P. et al. FTIR spectroscopy suggests a revised mode of action for the cationic side-chain effect of ionic liquids. ACS Comb. Sci. 21, 90\u201397 (2019).","journal-title":"ACS Comb. Sci."},{"key":"82308_CR45","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.vibspec.2016.09.016","volume":"91","author":"RJ Tidy","year":"2017","unstructured":"Tidy, R. J., Lam, V., Fimognari, N., Mamo, J. C. & Hackett, M. J. FTIR studies of the similarities between pathology induced protein aggregation in vivo and chemically induced protein aggregation ex vivo. Vib. Spectrosc. 91, 68\u201376 (2017).","journal-title":"Vib. Spectrosc."},{"key":"82308_CR46","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1111\/j.1600-065X.2008.00681.x","volume":"225","author":"JD Fraser","year":"2008","unstructured":"Fraser, J. D. & Proft, T. The bacterial superantigen and superantigen-like proteins. Immunol. Rev. 225, 226\u2013243 (2008).","journal-title":"Immunol. Rev."},{"key":"82308_CR47","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/BF00817929","volume":"9","author":"IF Cheng","year":"1996","unstructured":"Cheng, I. F., Zhao, C. P., Amolins, A., Galazka, M. & Doneski, L. A hypothesis for the in vivo antioxidant action of salicyclic acid. Biometals 9, 285\u2013290 (1996).","journal-title":"Biometals"},{"key":"82308_CR48","doi-asserted-by":"publisher","first-page":"19145","DOI":"10.1073\/pnas.1902752116","volume":"116","author":"SE George","year":"2019","unstructured":"George, S. E. et al. Oxidative stress drives the selection of quorum sensing mutants in the Staphylococcus aureus population. Proc. Natl. Acad. Sci. 116, 19145\u201319154 (2019).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"82308_CR49","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1111\/j.1574-6968.2010.02164.x","volume":"315","author":"JE Arce Miranda","year":"2011","unstructured":"Arce Miranda, J. E., Sotomayor, C. E., Albesa, I. & Paraje, M. G. Oxidative and nitrosative stress in Staphylococcus aureus biofilm. FEMS Microbiol. Lett. 315, 23\u201329 (2011).","journal-title":"FEMS Microbiol. Lett."},{"key":"82308_CR50","doi-asserted-by":"publisher","first-page":"5456","DOI":"10.1073\/pnas.92.12.5456","volume":"92","author":"RG Martin","year":"1995","unstructured":"Martin, R. G. & Rosner, J. L. Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences. Proc. Natl. Acad. Sci. 92, 5456\u20135460 (1995).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"82308_CR51","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1016\/j.chembiol.2011.05.014","volume":"18","author":"F Sun","year":"2011","unstructured":"Sun, F. et al. Targeting MgrA-mediated virulence regulation in Staphylococcus aureus. Chem. Biol. 18, 1032\u20131041 (2011).","journal-title":"Chem. Biol."},{"key":"82308_CR52","doi-asserted-by":"publisher","first-page":"7549","DOI":"10.1128\/JB.186.22.7549-7555.2004","volume":"186","author":"R Koenig","year":"2004","unstructured":"Koenig, R., Ray, J., Maleki, S., Smeltzer, M. & Hurlburt, B. Staphylococcus aureus AgrA binding to the RNAIII-agr regulatory region. J. Bacteriol. 186, 7549\u20137555 (2004).","journal-title":"J. Bacteriol."},{"issue":"5","key":"82308_CR53","doi-asserted-by":"publisher","first-page":"e0125027","DOI":"10.1371\/journal.pone.0125027","volume":"10","author":"BO Elmore","year":"2015","unstructured":"Elmore, B. O., Triplett, K. D. & Hall, P. R. Apolipoprotein B48, the structural component of chylomicrons, is sufficient to antagonize Staphylococcus aureus quorum sensing. PLoS ONE 10(5), e0125027 (2015).","journal-title":"PLoS ONE"},{"issue":"2","key":"82308_CR54","doi-asserted-by":"publisher","first-page":"e1003166","DOI":"10.1371\/journal.ppat.1003166","volume":"9","author":"PR Hall","year":"2013","unstructured":"Hall, P. R. et al. Nox2 modification of LDL is essential for optimal apolipoprotein B-mediated control of agr type III Staphylococcus aureus quorum-sensing. PLoS Pathog. 9(2), e1003166 (2013).","journal-title":"PLoS Pathog."},{"issue":"6","key":"82308_CR55","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/j.chom.2008.10.001","volume":"4","author":"MM Peterson","year":"2008","unstructured":"Peterson, M. M. et al. Apolipoprotein B is an innate barrier against invasive Staphylococcus aureus infection. Cell Host Microbe 4(6), 555\u2013566 (2008).","journal-title":"Cell Host Microbe"},{"key":"82308_CR56","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3389\/fcimb.2018.00018","volume":"8","author":"CM Suligoy","year":"2018","unstructured":"Suligoy, C. M. et al. Mutation of Agr is associated with the adaptation of Staphylococcus aureus to the host during chronic osteomyelitis. Front. Cell. Infect. Microbiol. 8, 18 (2018).","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"82308_CR57","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1080\/14728222.2018.1527314","volume":"22","author":"P Zimmermann","year":"2018","unstructured":"Zimmermann, P. & Curtis, N. The effect of aspirin on antibiotic susceptibility. J. Expert. Opin. Ther. Targets 22, 967\u2013972 (2018).","journal-title":"J. Expert. Opin. Ther. Targets"},{"key":"82308_CR58","doi-asserted-by":"publisher","first-page":"257","DOI":"10.3390\/molecules23020257","volume":"23","author":"F Soukarieh","year":"2018","unstructured":"Soukarieh, F. et al. In silico and in vitro-guided identification of inhibitors of alkylquinolone-dependent quorum sensing in Pseudomonas aeruginosa. Molecules 23, 257 (2018).","journal-title":"Molecules"},{"issue":"12","key":"82308_CR59","doi-asserted-by":"publisher","first-page":"e115959","DOI":"10.1371\/journal.pone.0115959","volume":"9","author":"T Grunert","year":"2014","unstructured":"Grunert, T. et al. Deciphering host genotype-specific impacts on the metabolic fingerprint of Listeria monocytogenes by FTIR spectroscopy. PLoS ONE 9(12), e115959 (2014).","journal-title":"PLoS ONE"},{"issue":"4","key":"82308_CR60","doi-asserted-by":"publisher","first-page":"e00198","DOI":"10.1128\/mBio.00198-12","volume":"3","author":"JB Kaplan","year":"2012","unstructured":"Kaplan, J. B. et al. Low levels of \u03b2-lactam antibiotics induce extracellular DNA release and biofilm formation in Staphylococcus aureus. mBio 3(4), e00198-e212 (2012).","journal-title":"mBio"},{"key":"82308_CR61","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u2206\u2206CT method. Methods 25, 402\u2013408 (2001).","journal-title":"Methods"},{"key":"82308_CR62","unstructured":"Gibb, S. MALDIquantForeign: import\/export routines for MALDIquant. At, https:\/\/cran.r-project.org\/package=MALDIquantForeign (2015)."},{"key":"82308_CR63","doi-asserted-by":"publisher","first-page":"2270","DOI":"10.1093\/bioinformatics\/bts447","volume":"28","author":"S Gibb","year":"2012","unstructured":"Gibb, S. & Strimmer, K. MALDIquant: a versatile R package for the analysis of mass spectrometry data. Bioinformatics 28, 2270\u20132271 (2012).","journal-title":"Bioinformatics"},{"key":"82308_CR64","unstructured":"Gibb, S. & Strimmer, K. Binda: multi-class discriminant analysis using binary predictors. R package version 1.0.3. At https:\/\/CRAN.R-project.org\/package=binda (2015)."},{"key":"82308_CR65","unstructured":"Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https:\/\/imagej.nih.gov\/ij\/, 1997\u20132018."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-82308-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-82308-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-82308-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:35:09Z","timestamp":1697762109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-82308-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,3]]},"references-count":65,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["82308"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-82308-y","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,3]]},"assertion":[{"value":"1 July 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 January 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2953"}}