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Despite the recommendation by WHO and OIE to use the direct immunofluorescence test as standard method, molecular diagnostic assays like reverse transcription quantitative polymerase chain reaction (RT-qPCR) are increasing as a confirmatory method. However, both technologies are inaccessible in resource-limited settings. Moreover, the available point-of-need molecular assay is of poor detection limit for African strains. Herein, we developed a reverse transcription recombinase polymerase amplification (RT-RPA) assay as potential point-of-need diagnostic tool for rapid detection of various strains of rabies virus including locally isolated African strains. The sensitivity and specificity of the method was evaluated using a molecular RNA standard and different Rabies-related viruses belonging to the\n                    <jats:italic>Rhabdoviridea<\/jats:italic>\n                    family, respectively. The RABV-RPA performances were evaluated on isolates representative of the existing diversity and viral dilutions spiked in non-neural clinical specimen. The results were compared with RT-qPCR as a gold standard. The RABV-RPA detected down to 4 RNA molecules per reaction in 95% of the cases in less than 10\u00a0min. The RABV-RPA assay is highly specific as various RABV isolates were identified, but no amplification was observed for other member of the\n                    <jats:italic>Rhabdoviridea family<\/jats:italic>\n                    . The sample background did not affect the performance of the RABV-RPA as down to 11 RNA molecules were identified, which is similar to the RT-qPCR results. Our developed assay is suitable for use in low-resource settings as a promising alternative tool for\n                    <jats:italic>ante-mortem<\/jats:italic>\n                    rabies diagnosis in humans for facilitating timely control decisions.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-82479-8","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T06:08:10Z","timestamp":1612418890000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A recombinase polymerase amplification assay for rapid detection of rabies virus"],"prefix":"10.1038","volume":"11","author":[{"given":"Martin","family":"Faye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abd El Wahed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumar","family":"Faye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Kissenk\u00f6tter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou Alpha","family":"Sall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousmane","family":"Faye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"issue":"4","key":"82479_CR1","doi-asserted-by":"publisher","first-page":"e2057","DOI":"10.1371\/journal.pone.0002057","volume":"3","author":"O Delmas","year":"2008","unstructured":"Delmas, O. et al. 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