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However, mechanisms mediating association between these two conditions remain unclear. This study investigated, whether the OSA-associated changes in adipose tissue lipolysis might contribute to impaired glucose homeostasis in patient with T2DM. Thirty-five matched subjects were recruited into three groups: T2DM\u2009+\u2009severe OSA (T2DM\u2009+\u2009OSA, n\u2009=\u200911), T2DM with mild\/no OSA (T2DM, n\u2009=\u200910) and healthy controls (n\u2009=\u200914). Subcutaneous abdominal adipose tissue microdialysis assessed spontaneous, epinephrine- and isoprenaline-stimulated lipolysis. Glucose metabolism was assessed by intravenous glucose tolerance test. Spontaneous lipolysis was higher in the T2DM\u2009+\u2009OSA compared with the T2DM (60.34\u2009\u00b1\u200923.40 vs. 42.53\u2009\u00b1\u200910.16\u00a0\u03bcmol\/L, <jats:italic>p<\/jats:italic>\u2009=\u20090.013), as well as epinephrine-stimulated lipolysis (236.84\u2009\u00b1\u2009103.90 vs. 167.39\u2009\u00b1\u200952.17\u00a0\u00b5mol\/L, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Isoprenaline-stimulated lipolysis was unaffected by the presence of OSA (<jats:italic>p<\/jats:italic>\u2009=\u20090.750). The \u03b1<jats:sub>2<\/jats:sub> anti-lipolytic effect was decreased in T2DM\u2009+\u2009OSA by 59% and 315% compared with T2DM and controls (<jats:italic>p<\/jats:italic>\u2009=\u20090.045 and <jats:italic>p<\/jats:italic>\u2009=\u20090.007, respectively). The severity of OSA (AHI) was positively associated with spontaneous (<jats:italic>p<\/jats:italic>\u2009=\u20090.037) and epinephrine-stimulated (<jats:italic>p<\/jats:italic>\u2009=\u20090.026) lipolysis. The \u03b1<jats:sub>2<\/jats:sub>-adrenergic anti-lipolytic effect (<jats:italic>p<\/jats:italic>\u2009=\u20090.043) decreased with increasing AHI. Spontaneous lipolysis was positively associated with Insulin resistance (r\u2009=\u20090.50, <jats:italic>p<\/jats:italic>\u2009=\u20090.002). Epinephrine-stimulated lipolysis was negatively associated with the Disposition index (r\u2009=\u2009\u2009\u2212\u20090.34, <jats:italic>p<\/jats:italic>\u2009=\u20090.048). AHI was positively associated with Insulin resistance (<jats:italic>p<\/jats:italic>\u2009=\u20090.017) and negatively with the Disposition index (<jats:italic>p<\/jats:italic>\u2009=\u20090.038). Severe OSA in patients with T2DM increased adipose tissue lipolysis, probably due to inhibition of the \u03b1<jats:sub>2<\/jats:sub>-adrenergic anti-lipolytic effect. We suggest that dysregulated lipolysis might contribute to OSA-associated impairments in insulin secretion and sensitivity.<\/jats:p>","DOI":"10.1038\/s41598-021-83018-1","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T11:05:48Z","timestamp":1613041548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Obstructive sleep apnoea increases lipolysis and deteriorates glucose homeostasis in patients with type 2 diabetes mellitus"],"prefix":"10.1038","volume":"11","author":[{"given":"Minh Duc","family":"Trinh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Plihalova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Gojda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Westlake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Spicka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Lattova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Pretl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Polak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"83018_CR1","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1038\/nrendo.2017.151","volume":"14","author":"Y Zheng","year":"2017","unstructured":"Zheng, Y., Ley, S. 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