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For this we loaded the RBCs with the fluorinated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-\n                    <jats:italic>N,N,N\u2032,N\u2032<\/jats:italic>\n                    -tetraacetic acid (5FBAPTA), and recorded\n                    <jats:sup>19<\/jats:sup>\n                    F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching\/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and\n                    <jats:sup>13<\/jats:sup>\n                    C NMR spectroscopy with [1,6-\n                    <jats:sup>13<\/jats:sup>\n                    C]\n                    <jats:sc>d<\/jats:sc>\n                    -glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx is concluded to be via the mechanosensitive cation channel Piezo1. The increased rate of influx brought about by the activator of Piezo1, 2-[5-[[(2,6-dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]-pyrazine (Yoda1\n                    <jats:bold>)<\/jats:bold>\n                    supported this conclusion; while the specificity of the cation-sensing by 5FBAPTA was confirmed by using the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ionophore, A23187.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-83044-z","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T06:07:44Z","timestamp":1613110064000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy"],"prefix":"10.1038","volume":"11","author":[{"given":"Philip W.","family":"Kuchel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Romanenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Shishmarev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petrik","family":"Galvosas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles D.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"issue":"10","key":"83044_CR1","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1126\/sciadv.aao1016","volume":"3","author":"PW Kuchel","year":"2017","unstructured":"Kuchel, P. 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