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In this work, ethanol and ethanol\u2013water extracts of air-dried and lyophilized flowers of <jats:italic>H. perforatum<\/jats:italic> L., collected throughout a vegetation season in central Poland, were studied. Air-dried flowers extracts had higher polyphenol (371\u00a0mg GAE\/g) and flavonoid (160\u00a0mg CAE\/g) content, DPPH radical scavenging (1672\u00a0mg DPPH\/g), ORAC (5214\u00a0\u00b5mol TE\/g) and FRAP (2.54\u00a0mmol Fe<jats:sup>2+<\/jats:sup>\/g) than lyophilized flowers extracts (238\u00a0mg GAE\/g, 107\u00a0mg CAE\/g, 1287\u00a0mg DPPH\/g, 3313\u00a0\u00b5mol TE\/g and 0.31\u00a0mmol Fe<jats:sup>2+<\/jats:sup>\/g, respectively). Principal component analysis showed that the collection date influenced the flavonoid and polyphenol contents and FRAP of ethanol extracts, and DPPH and ORAC values of ethanol\u2013water extracts. The ethanol extracts with the highest polyphenol and flavonoid content protected human erythrocytes against bisphenol A-induced damage. Both high field and benchtop NMR spectra of selected extracts, revealed differences in composition caused by extraction solvent and raw material collection date. Moreover, we have shown that benchtop NMR can be used to detect the compositional variation of extracts if the assignment of signals is done previously.<\/jats:p>","DOI":"10.1038\/s41598-021-83409-4","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T16:46:37Z","timestamp":1613753197000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John\u2019s wort\u2019s (Hypericum perforatum L.) flowers"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8660-9631","authenticated-orcid":false,"given":"Katerina","family":"Makarova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna J.","family":"Sajkowska-Kozielewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Zawada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Olchowik-Grabarek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142 Aleksander","family":"Ciach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Gogolewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Dobros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Ciechowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Freichels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gambin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"83409_CR1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1136\/bmj.313.7052.253","volume":"313","author":"K Linde","year":"1996","unstructured":"Linde, K. et al. 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Dr H\u00e9l\u00e8ne Freichels works at Magritek as sales representative, and she performed the benchtop NMR experiments on their equipment for this work and helped to interpret the NMR results. The rest of the authors have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3989"}}