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This work aims to quantify the delivery of the monoclonal antibody trastuzumab to rat spinal cord tissue and characterize its distribution within a model of leptomeningeal metastases. 10 healthy Sprague\u2013Dawley rats were treated with FUS\u2009+\u2009trastuzumab and sacrificed at 2-h or 24-h post-FUS. A human IgG ELISA (Abcam) was used to measure trastuzumab concentration and a 12\u2009\u00b1\u2009fivefold increase was seen in treated tissue over control tissue at 2\u00a0h versus no increase at 24\u00a0h. Three athymic nude rats were inoculated with MDA-MB-231-H2N HER2\u2009+\u2009breast cancer cells between the meninges in the thoracic region of the spinal cord and treated with FUS\u2009+\u2009trastuzumab. Immunohistochemistry was performed to visualize trastuzumab delivery, and semi-quantitative analysis revealed similar or more intense staining in tumor tissue compared to healthy tissue suggesting a comparable or greater concentration of trastuzumab was achieved. FUS can increase the permeability of the BSCB, improving drug delivery to specifically targeted regions of healthy and pathologic tissue in the spinal cord. The achieved concentrations within the healthy tissue are comparable to those reported in the brain.<\/jats:p>","DOI":"10.1038\/s41598-021-83874-x","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T11:03:35Z","timestamp":1614164615000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of ultrasound-mediated delivery of trastuzumab to normal and pathologic spinal cord tissue"],"prefix":"10.1038","volume":"11","author":[{"given":"Paige","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Ogrodnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janani","family":"Satkunarajah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meaghan A.","family":"O\u2019Reilly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,24]]},"reference":[{"key":"83874_CR1","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1002\/ana.22421","volume":"70","author":"V Bartanusz","year":"2011","unstructured":"Bartanusz, V., Jezova, D., Alajajian, B. & Digicaylioglu, M. 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