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These infections are notoriously difficult to treat, and it is believed that the ability of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    to invade endothelial cells and persist intracellularly is a key mechanism for persistence despite ongoing antibiotic treatment. Here, we used dual RNA sequencing to study the simultaneous transcriptional response of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and human endothelial cells during in vitro infections. We revealed discrete and shared differentially expressed genes for both host and pathogen at the different stages of infection. While the endothelial cells upregulated genes involved in interferon signalling and antigen presentation during late infection,\n                    <jats:italic>S. aureus<\/jats:italic>\n                    downregulated toxin expression while upregulating genes related to iron scavenging. In conclusion, the presented data provide an important resource to facilitate functional investigations into host\u2013pathogen interaction during\n                    <jats:italic>S. aureus<\/jats:italic>\n                    invasive infection and a basis for identifying novel drug target sites.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-84050-x","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T07:14:42Z","timestamp":1615878882000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus"],"prefix":"10.1038","volume":"11","author":[{"given":"Rasmus Birkholm","family":"Gr\u00f8nnemose","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Garde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claes S\u00f8ndergaard","family":"Wassmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janne Kudsk","family":"Klitgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronni","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Mandrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas Holm","family":"Mattsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Emil","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,16]]},"reference":[{"key":"84050_CR1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.mib.2013.11.004","volume":"17","author":"M Otto","year":"2014","unstructured":"Otto, M. 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