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In the current work, we studied the effects of the <jats:italic>CLE<\/jats:italic> family genes on growth and development in cotton. Based on the presence of a conserved CLE motif of 13 amino acids, 93 genes were characterized as <jats:italic>GhCLE<\/jats:italic> gene family members, and these were subcategorized into 7 groups. A preliminary analysis of the cotton CLE gene family indicated that the activity of its members tends to be conserved in terms of both the 13-residue conserved domain at the C-terminus and their subcellular localization pattern. Among the 14 tested genes, the ectopic overexpression of <jats:italic>GhCLE5::GFP<\/jats:italic> partially mimicked the phenotype of the <jats:italic>clv3<\/jats:italic> mutant in <jats:italic>Arabidopsis<\/jats:italic>. GhCLE5 could affect the endogenous CLV3 in binding to the receptor complex, comprised of CLV1, CLV2, and CRN, in the yeast two-hybrid assay and split-luciferase assay. Silencing <jats:italic>GhCLE5<\/jats:italic> in cotton caused a short seedling phenotype. Therefore, we concluded that the cotton <jats:italic>GhCLE<\/jats:italic> gene family is functionally conserved in apical shoot development regulation. These results indicate that CLE also plays roles in cotton development as a short peptide hormone.<\/jats:p>","DOI":"10.1038\/s41598-021-84312-8","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T11:02:36Z","timestamp":1614769356000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development"],"prefix":"10.1038","volume":"11","author":[{"given":"Ke","family":"Wan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kening","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengtao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Lei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyuan","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guosheng","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,3]]},"reference":[{"key":"84312_CR1","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/j.tplants.2016.07.002","volume":"21","author":"J Nardmann","year":"2016","unstructured":"Nardmann, J., Chandler, J. W. & Werr, W. Stem cell fate versus differentiation: the missing link. Trends Plant Sci. 21, 725\u2013727. https:\/\/doi.org\/10.1016\/j.tplants.2016.07.002 (2016).","journal-title":"Trends Plant Sci."},{"key":"84312_CR2","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1111\/nph.14348","volume":"216","author":"DM Goad","year":"2017","unstructured":"Goad, D. M., Zhu, C. & Kellogg, E. A. Comprehensive identification and clustering of CLV3\/ESR-related (CLE) genes in plants finds groups with potentially shared function. New Phytol. 216, 605\u2013616. https:\/\/doi.org\/10.1111\/nph.14348 (2017).","journal-title":"New Phytol."},{"key":"84312_CR3","doi-asserted-by":"publisher","first-page":"5271","DOI":"10.1093\/jxb\/erv351","volume":"66","author":"AH Hastwell","year":"2015","unstructured":"Hastwell, A. H., Gresshoff, P. M. & Ferguson, B. J. 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