{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:26:10Z","timestamp":1792790770355,"version":"4.5.0"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["KJZD-K201803501"],"award-info":[{"award-number":["KJZD-K201803501"]}],"id":[{"id":"https:\/\/ror.org\/031nm5713","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Neijiang City S"},{"name":"\u201cDouble Support Project\u201d fund of Sichuan Agricultural University","award":["03573018"],"award-info":[{"award-number":["03573018"]}],"id":[{"id":"https:\/\/ror.org\/0388c3403","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fish culture in paddy fields is a traditional aquaculture mode, which has a long history in East Asia. Large-scale loach (\n                    <jats:italic>Paramisgurnus dabryanus<\/jats:italic>\n                    ) fast growth is suitable for paddy fields aquaculture in China. The objective of this study was to identify differential expression genes (DEGs) in the brain, liver and muscle tissues between large (LG, top 5% of maximum total length) and small (SG, top 5% of minimum total length) groups using RNA-seq. In total, 150 fish were collected each week and 450 fish were collected at twelfth week from three paddy fields for all the experimental. Histological observation found that the muscle fibre diameter of LG loaches was greater than that of SG loaches. Transcriptome results revealed that the high expression genes (HEGs) in LG loaches (fold change\u2009\u2265\u20092,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) were mainly concentrated in metabolic pathways, such as \u201cThyroid hormone signalling pathway\u201d, \u201cCitrate cycle (TCA cycle)\u201d, \u201cCarbon metabolism\u201d, \u201cFatty acid metabolism\u201d, and \u201cCholesterol metabolism\u201d, and the HEGs in SG loaches were enriched in the pathways related to environmental information processing such as \u201cCell adhesion molecules (CAMs)\u201d, \u201cECM\u2212\u2009receptor interaction\u201d and \u201cRap1 signalling pathway\u201d; cellular processes such as \u201cTight junction\u201d, \u201cFocal adhesion\u201d, \u201cPhagosome\u201d and \u201cAdherens junction\u201d. Furthermore, IGFs gene family may play an important role in loach growth for their different expression pattern between the two groups. These findings can enhance our understanding about the molecular mechanism of different growth and development levels of loaches in paddy fields.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-84519-9","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T06:04:56Z","timestamp":1614665096000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparative transcriptome profiles of large and small bodied large-scale loaches cultivated in paddy fields"],"prefix":"10.1038","volume":"11","author":[{"given":"Liulan","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xugan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"issue":"1\u20134","key":"84519_CR1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/S0925-8574(98)00022-6","volume":"11","author":"J Liu","year":"1998","unstructured":"Liu, J. & Cai, Q. Integrated aquaculture in Chinese lakes and paddy fields. Ecol. Eng. 11(1\u20134), 49\u201359 (1998).","journal-title":"Ecol. Eng."},{"key":"84519_CR2","first-page":"26","volume":"10","author":"SG Lu","year":"1988","unstructured":"Lu, S. G. & Huang, C. P. Economic and ecological effects of fish culture in paddy fields. Chin. J. Ecol. 10, 26 (1988).","journal-title":"Chin. J. Ecol."},{"key":"84519_CR3","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/978-90-481-3087-0_2","volume-title":"Success stories in Asian aquaculture","author":"M Weimin","year":"2010","unstructured":"Weimin, M. Recent developments in rice-fish culture in China: A holistic approach for livelihood improvement in rural areas. In Success stories in Asian aquaculture 15\u201340 (Springer, Berlin, 2010)."},{"issue":"2","key":"84519_CR4","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.gene.2014.12.034","volume":"557","author":"C Li","year":"2015","unstructured":"Li, C. et al. Transcriptome characterization and SSR discovery in large-scale loach Paramisgurnus dabryanus (Cobitidae, Cypriniformes). Gene 557(2), 201\u2013208 (2015).","journal-title":"Gene"},{"issue":"1","key":"84519_CR5","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s10641-007-9282-x","volume":"86","author":"C You","year":"2009","unstructured":"You, C., Yu, X. & Tong, J. Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations. Environ. Biol. Fishes 86(1), 65 (2009).","journal-title":"Environ. Biol. Fishes"},{"key":"84519_CR6","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.fsi.2017.01.008","volume":"62","author":"Y Gao","year":"2017","unstructured":"Gao, Y. et al. Effects of fulvic acid on growth performance and intestinal health of juvenile loach Paramisgurnus dabryanus (Sauvage). Fish Shellfish Immunol. 62, 47\u201356 (2017).","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"84519_CR7","first-page":"84","volume":"33","author":"C-H You","year":"2012","unstructured":"You, C.-H., Tong, J.-G. & Yu, X.-M. Microsatellite DNA analysis on genetic diversity of seven populations of Paramisgurnus dabryanus. J. Hydroecol. 33(1), 84\u201391 (2012).","journal-title":"J. Hydroecol."},{"issue":"1","key":"84519_CR8","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1111\/j.1095-8649.1987.tb05213.x","volume":"31","author":"C Talesara","year":"1987","unstructured":"Talesara, C. & Urfi, A. A histophysiological study of muscle differentiation and growth in the common carp Cyprinus carpio Var. communis. J. Fish Biol. 31(1), 45\u201354 (1987).","journal-title":"J. Fish Biol."},{"issue":"16","key":"84519_CR9","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1242\/jeb.205.16.2349","volume":"205","author":"W Stoiber","year":"2002","unstructured":"Stoiber, W. et al. Cellularity changes in developing red and white fish muscle at different temperatures: Simulating natural environmental conditions for a temperate freshwater cyprinid. J. Exp. Biol. 205(16), 2349\u20132364 (2002).","journal-title":"J. Exp. Biol."},{"issue":"4","key":"84519_CR10","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/S0960-9822(01)00068-9","volume":"11","author":"W Brogiolo","year":"2001","unstructured":"Brogiolo, W. et al. An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control. Curr. Biol. 11(4), 213\u2013221 (2001).","journal-title":"Curr. Biol."},{"issue":"22","key":"84519_CR11","doi-asserted-by":"publisher","first-page":"3895","DOI":"10.1242\/jeb.068908","volume":"215","author":"IP Amaral","year":"2012","unstructured":"Amaral, I. P. & Johnston, I. A. Experimental selection for body size at age modifies early life-history traits and muscle gene expression in adult zebrafish. J. Exp. Biol. 215(22), 3895\u20133904 (2012).","journal-title":"J. Exp. Biol."},{"issue":"6","key":"84519_CR12","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.1007\/s10126-011-9376-5","volume":"13","author":"M Van der Merwe","year":"2011","unstructured":"Van der Merwe, M., Franchini, P. & Roodt-Wilding, R. Differential growth-related gene expression in abalone (Haliotis midae). Mar. Biotechnol. 13(6), 1125\u20131139 (2011).","journal-title":"Mar. Biotechnol."},{"issue":"2","key":"84519_CR13","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.tibtech.2005.12.008","volume":"24","author":"RH Devlin","year":"2006","unstructured":"Devlin, R. H., Sundstr\u00f6m, L. F. & Muir, W. M. Interface of biotechnology and ecology for environmental risk assessments of transgenic fish. Trends Biotechnol. 24(2), 89\u201397 (2006).","journal-title":"Trends Biotechnol."},{"issue":"1","key":"84519_CR14","first-page":"R97","volume":"301","author":"I Seiliez","year":"2011","unstructured":"Seiliez, I., Sabin, N. & Gabillard, J.-C. FoxO1 is not a key transcription factor in the regulation of myostatin (mstn-1a and mstn-1b) gene expression in trout myotubes. Am. J. Physiol. 301(1), R97\u2013R104 (2011).","journal-title":"Am. J. Physiol."},{"issue":"3","key":"84519_CR15","first-page":"395","volume":"49","author":"C Darwin","year":"1859","unstructured":"Darwin, C. On the origin of species by means of natural selection. Am. Midland Nat. 49(3), 395\u2013405 (1859).","journal-title":"Am. Midland Nat."},{"key":"84519_CR16","first-page":"33","volume":"3","author":"T Gjedrem","year":"1997","unstructured":"Gjedrem, T. Contribution from selective breeding to future aquaculture development. J. World Aquacult. Soc. 3, 33\u201345 (1997).","journal-title":"J. World Aquacult. Soc."},{"issue":"2","key":"84519_CR17","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1007\/s00429-002-0232-2","volume":"205","author":"W Stoiber","year":"2002","unstructured":"Stoiber, W. et al. Tonic fibres in axial muscle of cyprinid fish larvae: their definition, possible origins and functional importance. Anat. Embryol. 205(2), 113\u2013124 (2002).","journal-title":"Anat. Embryol."},{"issue":"5","key":"84519_CR18","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1051\/rnd:2002035","volume":"42","author":"B Picard","year":"2002","unstructured":"Picard, B., Lefaucheur, L., Berri, C. C. & Duclos, M. J. Muscle fibre ontogenesis in farm animal species. Reprod. Nutr. Dev. 42(5), 415\u2013431 (2002).","journal-title":"Reprod. Nutr. Dev."},{"issue":"6","key":"84519_CR19","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/nrm3118","volume":"12","author":"T Braun","year":"2011","unstructured":"Braun, T. & Gautel, M. Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat. Rev. Mol. Cell Biol. 12(6), 349 (2011).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"6","key":"84519_CR20","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1111\/j.1095-8649.1997.tb01653.x","volume":"50","author":"H Alami-Durante","year":"1997","unstructured":"Alami-Durante, H. et al. Growth and multiplication of white skeletal muscle fibres in carp larvae in relation to somatic growth rate. J. Fish Biol. 50(6), 1285\u20131302 (1997).","journal-title":"J. Fish Biol."},{"issue":"1","key":"84519_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00187522","volume":"182","author":"A Veggetti","year":"1990","unstructured":"Veggetti, A., Mascarello, F., Scapolo, P. A. & Rowlerson, A. Hyperplastic and hypertrophic growth of lateral muscle in Dicentrarchus labrax (L.). Anat. Embryol. 182(1), 1\u201310 (1990).","journal-title":"Anat. Embryol."},{"issue":"4","key":"84519_CR22","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1007\/BF00185893","volume":"187","author":"A Veggetti","year":"1993","unstructured":"Veggetti, A. et al. Muscle growth and myosin isoform transitions during development of a small teleost fish, Poecilia reticulata (Peters) (Atheriniformes, Poeciliidae): A histochemical, immunohistochemical, ultrastructural and morphometric study. Anat. Embryol. 187(4), 353\u2013361 (1993).","journal-title":"Anat. Embryol."},{"issue":"14","key":"84519_CR23","doi-asserted-by":"publisher","first-page":"8227","DOI":"10.1021\/es502366g","volume":"48","author":"YF Zhang","year":"2014","unstructured":"Zhang, Y. F. et al. Tetrabromobisphenol A disrupts vertebrate development via thyroid hormone signaling pathway in a developmental stage-dependent manner. Environ. Ence Technol. 48(14), 8227\u20138234 (2014).","journal-title":"Environ. Ence Technol."},{"issue":"6 Pt 1","key":"84519_CR24","first-page":"E1130","volume":"277","author":"DE Kelley","year":"1999","unstructured":"Kelley, D. E., Goodpaster, B., Wing, R. R. & Simoneau, J. A. Skeletal muscle fatty acid metabolism in association with insulin resistance, obesity, and weight loss. Am. J. Physiol. 277(6 Pt 1), E1130\u2013E1141 (1999).","journal-title":"Am. J. Physiol."},{"issue":"8","key":"84519_CR25","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1194\/jlr.R400004-JLR200","volume":"45","author":"JM Dietschy","year":"2004","unstructured":"Dietschy, J. M. & Turley, S. D. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J. Lipid Res. 45(8), 1375\u20131397 (2004).","journal-title":"J. Lipid Res."},{"issue":"1","key":"84519_CR26","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1186\/1471-2164-15-57","volume":"15","author":"AL Kocmarek","year":"2014","unstructured":"Kocmarek, A. L., Ferguson, M. M. & Danzmann, R. G. Differential gene expression in small and large rainbow trout derived from two seasonal spawning groups. BMC Genom. 15(1), 57 (2014).","journal-title":"BMC Genom."},{"issue":"9","key":"84519_CR27","doi-asserted-by":"publisher","first-page":"3047","DOI":"10.1073\/pnas.0809798106","volume":"106","author":"RH Devlin","year":"2009","unstructured":"Devlin, R. H., Sakhrani, D., Tymchuk, W. E., Rise, M. L. & Goh, B. Domestication and growth hormone transgenesis cause similar changes in gene expression in coho salmon (Oncorhynchus kisutch). Proc. Natl. Acad. Sci. USA 106(9), 3047\u20133052 (2009).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"2","key":"84519_CR28","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1677\/jme.1.02031","volume":"37","author":"ML Rise","year":"2006","unstructured":"Rise, M. L. et al. Multiple microarray platforms utilized for hepatic gene expression profiling of GH transgenic coho salmon with and without ration restriction. J. Mol. Endocrinol. 37(2), 259\u2013282 (2006).","journal-title":"J. Mol. Endocrinol."},{"key":"84519_CR29","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1186\/1471-2164-14-673","volume":"14","author":"SL White","year":"2013","unstructured":"White, S. L., Sakhrani, D., Danzmann, R. G. & Devlin, R. H. Influence of developmental stage and genotype on liver mRNA levels among wild, domesticated, and hybrid rainbow trout (Oncorhynchus mykiss). Bmc Genom. 14, 673 (2013).","journal-title":"Bmc Genom."},{"issue":"12","key":"84519_CR30","doi-asserted-by":"publisher","first-page":"4266","DOI":"10.2527\/jas.2012-5142","volume":"90","author":"CW Huang","year":"2012","unstructured":"Huang, C. W. et al. Differential expression patterns of growth-related microRNAs in the skeletal muscle of Nile tilapia. J. Anim. Sci. 90(12), 4266\u20134279 (2012).","journal-title":"J. Anim. Sci."},{"issue":"2","key":"84519_CR31","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1577\/A07-031.1","volume":"70","author":"BC Small","year":"2008","unstructured":"Small, B. C., Davis, K. B. & Peterson, B. C. Elucidating the effects of cortisol and stress on economically important traits in channel catfish. North Am. J. Aquacult. 70(2), 223\u2013235 (2008).","journal-title":"North Am. J. Aquacult."},{"issue":"15","key":"84519_CR32","doi-asserted-by":"publisher","first-page":"3167","DOI":"10.1111\/j.1365-294X.2011.05172.x","volume":"20","author":"DJ Macqueen","year":"2011","unstructured":"Macqueen, D. J. et al. The parallel evolution of dwarfism in Arctic charr is accompanied by adaptive divergence in mTOR-pathway gene expression. Mol. Ecol. 20(15), 3167\u20133184 (2011).","journal-title":"Mol. Ecol."},{"issue":"13","key":"84519_CR33","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1242\/jeb.053298","volume":"214","author":"IPG Amaral","year":"2011","unstructured":"Amaral, I. P. G. & Johnston, I. A. Insulin-like growth factor (IGF) signalling and genome-wide transcriptional regulation in fast muscle of zebrafish following a single-satiating meal. J. Exp. Biol. 214(13), 2125\u20132139 (2011).","journal-title":"J. Exp. Biol."},{"issue":"2","key":"84519_CR34","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1152\/physiolgenomics.00065.2010","volume":"42A","author":"NI Bower","year":"2010","unstructured":"Bower, N. I. & Johnston, I. A. Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon. Physiol. Genom. 42A(2), 114\u2013130 (2010).","journal-title":"Physiol. Genom."},{"issue":"1","key":"84519_CR35","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3181\/00379727-204-43630","volume":"204","author":"PD Lee","year":"1993","unstructured":"Lee, P. D., Conover, C. A. & Powell, D. R. Regulation and function of insulin-like growth factor-binding protein-1. Proc. Soc. Exp. Biol. Med. 204(1), 4\u201329 (1993).","journal-title":"Proc. Soc. Exp. Biol. Med."},{"issue":"4","key":"84519_CR36","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1073\/pnas.0407443102","volume":"102","author":"S Kajimura","year":"2005","unstructured":"Kajimura, S., Aida, K. & Duan, C. Insulin-like growth factor-binding protein-1 (IGFBP-1) mediates hypoxia-induced embryonic growth and developmental retardation. Proc. Natl. Acad. Sci. USA 102(4), 1240\u20131245 (2005).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"84519_CR37","doi-asserted-by":"publisher","first-page":"e3091","DOI":"10.1371\/journal.pone.0003091","volume":"3","author":"H Kamei","year":"2008","unstructured":"Kamei, H. et al. Duplication and diversification of the hypoxia-inducible IGFBP-1 gene in zebrafish. PLoS ONE 3, e3091 (2008).","journal-title":"PLoS ONE"},{"issue":"5","key":"84519_CR38","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.annonc.2020.01.066","volume":"31","author":"N Murphy","year":"2020","unstructured":"Murphy, N. et al. Insulin-like growth factor-1, insulin-like growth factor-binding protein-3, and breast cancer risk: Observational and Mendelian randomization analyses with 430 000 women. Ann. Oncol. 31(5), 641\u2013649 (2020).","journal-title":"Ann. Oncol."},{"issue":"3","key":"84519_CR39","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1002\/sctm.19-0281","volume":"9","author":"Z Lu","year":"2020","unstructured":"Lu, Z. et al. Reprogramming of human fibroblasts into osteoblasts by insulin-like growth factor-binding protein 7. Stem Cells Transl. Med. 9(3), 403\u2013415 (2020).","journal-title":"Stem Cells Transl. Med."},{"key":"84519_CR40","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1210\/endo-128-2-689","volume":"128","author":"LJ Murphy","year":"1991","unstructured":"Murphy, L. J., Seneviratne, C., Moreira, P. & Reid, R. E. Enhanced expression of insulin-like growth factor binding protein4 in the fasted rat: The effects of insulin and growth hormone administration. Endocrinology 128, 689\u2013696 (1991).","journal-title":"Endocrinology"},{"issue":"4","key":"84519_CR41","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1002\/ece3.509","volume":"3","author":"J Leips","year":"2013","unstructured":"Leips, J., Helen Rodd, F. & Travis, J. The adaptive significance of population differentiation in offspring size of the least killifish, Heterandria formosa. Ecol. Evol. 3(4), 948\u2013960 (2013).","journal-title":"Ecol. Evol."},{"issue":"3","key":"84519_CR42","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1006\/hbeh.2001.1707","volume":"40","author":"TG Pottinger","year":"2001","unstructured":"Pottinger, T. G. & Carrick, T. R. Stress responsiveness affects dominant-subordinate relationships in rainbow trout. Horm. Behav. 40(3), 419\u2013427 (2001).","journal-title":"Horm. Behav."},{"key":"84519_CR43","doi-asserted-by":"publisher","first-page":"162","DOI":"10.3389\/fgene.2018.00162","volume":"9","author":"B Petra","year":"2018","unstructured":"Petra, B. et al. Identification of central regulators of calcium signaling and ECM\u2013receptor interaction genetically associated with the progression and recurrence of atrial fibrillation. Front. Genet. 9, 162 (2018).","journal-title":"Front. Genet."},{"key":"84519_CR44","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.neurobiolaging.2020.04.017","volume":"94","author":"B Erbaba","year":"2020","unstructured":"Erbaba, B. et al. Zebrafish brain RNA sequencing reveals that cell adhesion molecules are critical in brain aging. Neurobiol. Aging 94, 164\u2013175 (2020).","journal-title":"Neurobiol. Aging"},{"issue":"2","key":"84519_CR45","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1074\/jbc.M117.815381","volume":"293","author":"CJ Ramos","year":"2018","unstructured":"Ramos, C. J., Lin, C., Liu, X. & Antonetti, D. A. The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability. J. Biol. Chem. 293(2), 717\u2013730 (2018).","journal-title":"J. Biol. Chem."},{"issue":"7","key":"84519_CR46","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/S0248-4900(00)01101-1","volume":"92","author":"V Petit","year":"2000","unstructured":"Petit, V. & Thiery, J. P. Focal adhesions: Structure and dynamics. Biol. Cell 92(7), 477\u2013494 (2000).","journal-title":"Biol. Cell"},{"issue":"2","key":"84519_CR47","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s10142-018-0643-7","volume":"19","author":"JL Sun","year":"2019","unstructured":"Sun, J. L. et al. Analysis of miRNA-seq in the liver of common carp (Cyprinus carpio L.) in response to different environmental temperatures. Funct. Integr. Genom. 19(2), 265\u2013280 (2019).","journal-title":"Funct. Integr. Genom."},{"issue":"7","key":"84519_CR48","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1038\/nbt.1883","volume":"29","author":"MG Grabherr","year":"2011","unstructured":"Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29(7), 644 (2011).","journal-title":"Nat. Biotechnol."},{"issue":"5","key":"84519_CR49","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1093\/bioinformatics\/btg034","volume":"19","author":"G Pertea","year":"2003","unstructured":"Pertea, G. et al. TIGR Gene Indices clustering tools (TGICL): A software system for fast clustering of large EST datasets. Bioinformatics 19(5), 651\u2013652 (2003).","journal-title":"Bioinformatics"},{"issue":"1","key":"84519_CR50","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","volume":"26","author":"MD Robinson","year":"2010","unstructured":"Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: A Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26(1), 139\u2013140 (2010).","journal-title":"Bioinformatics"},{"issue":"4","key":"84519_CR51","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT Method. Methods 25(4), 402\u2013408 (2001).","journal-title":"Methods"},{"key":"84519_CR52","doi-asserted-by":"publisher","first-page":"631","DOI":"10.3389\/fphys.2019.00631","volume":"10","author":"S Yang","year":"2019","unstructured":"Yang, S. et al. Mixed diets reduce the oxidative stress of common carp (Cyprinus carpio): Based on microRNA sequencing. Front. Physiol. 10, 631 (2019).","journal-title":"Front. Physiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-84519-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-84519-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-84519-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T23:42:21Z","timestamp":1670024541000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-84519-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,2]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["84519"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-84519-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,2]]},"assertion":[{"value":"18 February 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 March 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"4936"}}