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This ability of<jats:italic>B. miyamotoi<\/jats:italic>may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we constructed a membrane protein-encoding gene library of<jats:italic>B. miyamotoi<\/jats:italic>using<jats:italic>Borrelia garinii<\/jats:italic>strain HT59G, which shows a transformable and serum-susceptible phenotype. By screening the library, we found that<jats:italic>bom1093<\/jats:italic>and<jats:italic>bom1515<\/jats:italic>of<jats:italic>B. miyamotoi<\/jats:italic>provided a serum-resistant phenotype to the recipient<jats:italic>B. garinii<\/jats:italic>. These<jats:italic>B. miyamotoi<\/jats:italic>genes are predicted to encode P35-like antigen genes and are conserved among relapsing fever borreliae. Functional analysis revealed that BOM1093 bound to serum vitronectin and that the C-terminal region of BOM1093 was involved in the vitronectin-binding property. Importantly, the<jats:italic>B. garinii<\/jats:italic>transformant was not serum-resistant when the C terminus-truncated BOM1093 was expressed. We also observed that the depletion of vitronectin from human serum enhances the bactericidal activity of BOM1093 expressing<jats:italic>B. garinii<\/jats:italic>, and the survival rate of BOM1093 expressing<jats:italic>B. garinii<\/jats:italic>in vitronectin-depleted serum is enhanced by the addition of purified vitronectin. Our data suggests that<jats:italic>B. miyamotoi<\/jats:italic>utilize BOM1093-mediated binding to vitronectin as a mechanism of serum resistance.<\/jats:p>","DOI":"10.1038\/s41598-021-85069-w","type":"journal-article","created":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T11:03:14Z","timestamp":1615287794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi"],"prefix":"10.1038","volume":"11","author":[{"given":"Kozue","family":"Sato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Kumagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai","family":"Takano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Gaowa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle R.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Ohnishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Kawabata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,9]]},"reference":[{"key":"85069_CR1","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1099\/00207713-45-4-804","volume":"45","author":"M Fukunaga","year":"1995","unstructured":"Fukunaga, M. et al. 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