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Two distinct group 1 chaperonins operate in the organelles of mitochondria and apicoplasts, while group 2 chaperonins function in the cytosol. No structural information has been reported for any chaperonin from plasmodium. In this study, we describe the crystal structure of a double heptameric ring <jats:italic>Plasmodium falciparum<\/jats:italic> mitochondrial chaperonin 60 (Cpn60) bound with ATP, which differs significantly from any known crystal structure of chaperonin 60. The structure likely represents a unique intermediate state during conformational conversion from the closed state to the opened state. Three of the seven apical domains are highly dynamic while the equatorial domains form a stable ring. The structure implies large movements of the apical domain in the solution play a role in nucleotide-dependent regulation of substrate binding and folding. A unique 26\u201327 residue insertion in the equatorial domain of <jats:italic>Plasmodium falciparum<\/jats:italic> mitochondrial chaperonin greatly increases both inter-ring and intra-ring subunit\u2013subunit interactions. The present structure provides new insights into the mechanism of Cpn60 in chaperonin assembly and function.<\/jats:p>","DOI":"10.1038\/s41598-021-85197-3","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T11:03:41Z","timestamp":1615806221000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Crystal structure of P. falciparum Cpn60 bound to ATP reveals an open dynamic conformation before substrate binding"],"prefix":"10.1038","volume":"11","author":[{"given":"Brian","family":"Nguyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai Kwan","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dashuang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niraj H.","family":"Tolia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,15]]},"reference":[{"key":"85197_CR1","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.tibs.2015.07.009","volume":"41","author":"M Hayer-Hartl","year":"2016","unstructured":"Hayer-Hartl, M., Bracher, A. & Hartl, F. 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