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However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and cost-effective are required. We developed an immunodiagnostic test based on <jats:italic>S. scabiei<\/jats:italic> var. <jats:italic>nyctereutis<\/jats:italic> RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens\u2014a highly expressed hypothetical protein \u201cQR98_0091190,\u201d another mite allergen known as \u201cSMIPP-Cc,\u201d and an abundant \u201cvitellogenin-like protein\u201d\u2014were evaluated by western-blot analysis. A lateral flow immunoassay, using specific antibodies against the vitellogenin-like protein, successfully detected scabies in the skin flakes of <jats:italic>S. scabiei<\/jats:italic>-infected raccoon dogs. 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