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However, with changing climate JEV has the potential to emerge in novel temperate regions. Here, we have assessed the vector competence of the temperate mosquito\n                    <jats:italic>Culex pipiens f. pipiens<\/jats:italic>\n                    to vector JEV genotype III at temperatures representative of those experienced, or predicted in the future during the summer months, in the United Kingdom. Our results show that\n                    <jats:italic>Cx. pipiens<\/jats:italic>\n                    is susceptible to JEV infection at both temperatures. In addition, at 25\u00a0\u00b0C, JEV disseminated from the midgut and was recovered in saliva samples, indicating the potential for transmission. At a lower temperature, 20\u00a0\u00b0C, following an incubation period of fourteen days, there were reduced levels of JEV dissemination and virus was not detected in saliva samples. The virus present in the bodies of these mosquitoes was restricted to the posterior midgut as determined by microscopy and viable virus was successfully recovered. Apart from the influence on virus dissemination, mosquito mortality was significantly increased at the higher temperature. Overall, our results suggest that temperature is a critical factor for JEV vector competence and infected-mosquito survival. This may in turn influence the vectorial capacity of\n                    <jats:italic>Cx. pipiens<\/jats:italic>\n                    to vector JEV genotype III in temperate areas.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-85411-2","type":"journal-article","created":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T07:03:33Z","timestamp":1615964613000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Temperate conditions restrict Japanese encephalitis virus infection to the mid-gut and prevents systemic dissemination in Culex pipiens mosquitoes"],"prefix":"10.1038","volume":"11","author":[{"given":"Arran J.","family":"Folly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Dorey-Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"Hern\u00e1ndez-Triana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart","family":"Ackroyd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Vidana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian Z. 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