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This study evaluated the bioactivity-guided antiplasmodial efficacy and immunomodulatory effects of solvent fractions of <jats:italic>Diospyros mespiliformis<\/jats:italic> in mice infected with a susceptible strain of <jats:italic>Plasmodium berghei<\/jats:italic> (NK 65). The crude methanol extract of the stem of <jats:italic>D. mespiliformis<\/jats:italic> (DM) was partitioned between <jats:italic>n<\/jats:italic>-hexane, dichloromethane, ethyl acetate and methanol. Male Swiss mice (20\u2009\u00b1\u20092\u00a0g) infected with <jats:italic>P. berghei<\/jats:italic> were grouped and treated with vehicle (10\u00a0mL\/kg, control), Artemether lumefantrine (10\u00a0mg\/kg), 100, 200 and 400\u00a0mg\/kg of <jats:italic>n<\/jats:italic>-hexane, dichloromethane, ethyl acetate and methanol fractions of <jats:italic>D. mespiliformis<\/jats:italic> for seven days. Blood was obtained for heme and hemozoin contents while serum was obtained for inflammatory cytokines and immunoglobulins G and M assessments. Liver mitochondria were isolated for mitochondrial permeability transition (mPT), mitochondrial F<jats:sub>1<\/jats:sub>F<jats:sub>0<\/jats:sub> ATPase (mATPase) and lipid peroxidation (mLPO) assays. The GC\u2013MS was used to identify the compounds present in the most potent fraction. The dichloromethane fraction had the highest parasite clearance and improved hematological indices relative to the drug control. The heme values increased, while the hemozoin content significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) decreased compared with the drug control. The highest dose of HF and MF opened the mPT pore while the reversal effects of DF on mPT, mATPase and mLPO were dose-dependent. The levels of IgG, IgM and TNF\u03b1 in the DF group were significantly higher than the drug control, while the IL-1<jats:italic>\u03b2<\/jats:italic> and IL-6 values did not vary linearly with the dose. Lupeol and Stigmastan-3,5-diene were the most abundant phytochemicals in the DF. The outcome of this study showed that the DF has immunomodulatory effects in infected mice, reduced proliferation of the malaria parasite and thus protect liver cells.<\/jats:p>","DOI":"10.1038\/s41598-021-85790-6","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T11:06:58Z","timestamp":1616670418000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Studies on the mitochondrial, immunological and inflammatory effects of solvent fractions of Diospyros mespiliformis Hochst in Plasmodium berghei-infected mice"],"prefix":"10.1038","volume":"11","author":[{"given":"Oluwole Moses","family":"David","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Oludele","family":"Olanlokun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bisola Evelyn","family":"Owoniyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MoyinOluwa","family":"Ayeni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwakemi","family":"Ebenezer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil Anthony","family":"Koorbanally","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"85790_CR1","unstructured":"World Malaria Report 2005. 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