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The <jats:italic>P. falciparum<\/jats:italic> Formate Nitrite Transporter, <jats:italic>Pf<\/jats:italic>FNT, mediates <jats:sc>l<\/jats:sc>-lactate transport across the plasma membrane of <jats:italic>P. falciparum<\/jats:italic> parasites and has been validated as a drug target. The <jats:italic>T. gondii<\/jats:italic> genome encodes three FNTs that have been shown to transport <jats:sc>l<\/jats:sc>-lactate, and which are proposed to be the targets of several inhibitors of <jats:italic>T. gondii<\/jats:italic> proliferation. Here, we show that each of the <jats:italic>Tg<\/jats:italic>FNTs localize to the <jats:italic>T. gondii<\/jats:italic> plasma membrane and are capable of transporting <jats:sc>l<\/jats:sc>-lactate across it, with <jats:italic>Tg<\/jats:italic>FNT1 making the primary contribution to <jats:sc>l<\/jats:sc>-lactate transport during the disease-causing lytic cycle of the parasite. We use the <jats:italic>Xenopus<\/jats:italic> oocyte expression system to provide direct measurements of <jats:sc>l<\/jats:sc>-lactate transport via <jats:italic>Tg<\/jats:italic>FNT1. We undertake a genetic analysis of the importance of the <jats:italic>tgfnt<\/jats:italic> genes for parasite proliferation, and demonstrate that all three <jats:italic>tgfnt<\/jats:italic> genes can be disrupted individually and together without affecting the lytic cycle under in vitro culture conditions. Together, our experiments identify the major lactate transporter in the disease causing stage of <jats:italic>T. gondii<\/jats:italic>, and reveal that this transporter is not required for parasite proliferation, indicating that <jats:italic>Tg<\/jats:italic>FNTs are unlikely to be targets for anti-<jats:italic>Toxoplasma<\/jats:italic> drugs.<\/jats:p>","DOI":"10.1038\/s41598-021-86204-3","type":"journal-article","created":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T11:04:07Z","timestamp":1616583847000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identifying the major lactate transporter of Toxoplasma gondii tachyzoites"],"prefix":"10.1038","volume":"11","author":[{"given":"Joy M.","family":"Zeng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanduni V.","family":"Hapuarachchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah H.","family":"Shafik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rowena E.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiaran","family":"Kirk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giel G.","family":"van Dooren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele M.","family":"Lehane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,24]]},"reference":[{"key":"86204_CR1","unstructured":"W.H.O. 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