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In vitro activities were evaluated against <jats:italic>S. aureus<\/jats:italic> HG003 and <jats:italic>E. faecium<\/jats:italic> Aus0004. We determined MIC, MBC, sub-inhibitory concentrations (SIC) and growth curves under SIC conditions. sRNA expression study under SIC exposure of biocides was performed by RT-qPCR on 3 sRNAs expressed in <jats:italic>S. aureus<\/jats:italic> (RNAIII, SprD and SprX) and the first 9 sRNAs identified as expressed in <jats:italic>E. faecium<\/jats:italic>. MICs were higher against <jats:italic>E. faecium<\/jats:italic> than for <jats:italic>S. aureus<\/jats:italic>. Growth curves under increasing biocide concentrations highlighted two types of bactericidal activity: \u201con\/off\u201d effect for chlorhexidine, benzalkonium chloride, PVP-iodine and a \u201cconcentration-dependent\u201d activity for triclosan. Exposure to biocide SICs led to an alteration of several sRNA expression profiles, mostly repressed. The distinct biocide activity profiles must be evaluated with other compounds and bacterial species to enrich the prediction of resistance risks associated with biocide usage. Biocide exposure induces various sRNA-mediated responses in both <jats:italic>S. aureus<\/jats:italic> and <jats:italic>E. faecium<\/jats:italic>, and further investigations are needed to decipher sRNA-driven regulatory networks.<\/jats:p>","DOI":"10.1038\/s41598-021-86376-y","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T11:06:58Z","timestamp":1616670418000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Distinct expression profiles of regulatory RNAs in the response to biocides in Staphylococcus aureus and Enterococcus faecium"],"prefix":"10.1038","volume":"11","author":[{"given":"Loren","family":"Dejoies","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Killian","family":"Le Neindre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Reissier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice","family":"Felden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"86376_CR1","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1086\/533452","volume":"197","author":"LB Rice","year":"2008","unstructured":"Rice, L. 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