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The effects of SFE-CO2<\/jats:sub> parameters such as temperature (ranged from 40 to 70\u00a0$$^\\circ{\\rm C}$$<\/jats:tex-math>\n \n \n \n \u2218<\/mml:mo>\n <\/mml:msup>\n C<\/mml:mi>\n <\/mml:mrow>\n <\/mml:math><\/jats:alternatives><\/jats:inline-formula>), pressure (ranged from 100 to 350 bars) and dynamic time (from 70\u00a0min to 7\u00a0h) were analysed on the extraction efficiency of polyprenol. Qualitative and quantitative analysis of polyprenol was examined using high-performance liquid chromatography. Results showed that P. sylvestris<\/jats:italic> accumulated the highest polyprenol yield of 14.00\u00a0\u2009\u00b1\u2009$$0.4$$<\/jats:tex-math>\n \n 0.4<\/mml:mn>\n <\/mml:mrow>\n <\/mml:math><\/jats:alternatives><\/jats:inline-formula>mg\u00a0g\u22121<\/jats:sup> DW when extracted with hexane:acetone (1:1 v\/v). However, with SFE-CO2<\/jats:sub> conditions of 200 bars, 70\u00a0$$^\\circ{\\rm C}$$<\/jats:tex-math>\n \n \n \n \u2218<\/mml:mo>\n <\/mml:msup>\n C<\/mml:mi>\n <\/mml:mrow>\n <\/mml:math><\/jats:alternatives><\/jats:inline-formula>, 7\u00a0h, with absolute ethanol as a cosolvent with a flow rate of 0.05\u00a0ml min\u22121<\/jats:sup>, P. sitchensis<\/jats:italic> accumulated the highest polyprenol yield of 6.35\u2009\u00b1\u2009$$0.4$$<\/jats:tex-math>\n \n 0.4<\/mml:mn>\n <\/mml:mrow>\n <\/mml:math><\/jats:alternatives><\/jats:inline-formula> mg g\u22121<\/jats:sup>DW. This study emphasised the potential application of SFE-CO2<\/jats:sub> in the extraction of polyprenol as an environmentally friendly method to be used in pharmaceutical and food industries.<\/jats:p>","DOI":"10.1038\/s41598-021-86393-x","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T10:03:08Z","timestamp":1617357788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Supercritical-CO2 extraction, identification and quantification of polyprenol as a bioactive ingredient from Irish trees species"],"prefix":"10.1038","volume":"11","author":[{"given":"Hadil","family":"Alaydi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Downey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"McKeon-Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya","family":"Beletskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,2]]},"reference":[{"key":"86393_CR1","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1007\/s12257-018-0384-4","volume":"24","author":"EJ Kim","year":"2019","unstructured":"Kim, E. 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